Zebrafish as a genomics research model

Eleanor Chen, Stephen C Ekker

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The zebrafish (Danio rerio) is a recent addition to the genomic scientists' repertoire of vertebrate animal model systems. Unlike simple invertebrates such as the fly or the nematode, this teleost maintains the biological and genomic complexity found in higher vertebrates. Furthermore, the zebrafish has many advantageous technical and genomic properties that open the door to experimental approaches not practical using more classical models. The zebrafish genome can be functionally accessed using both forward and reverse genetics based approaches. A notable recent addition to the zebrafish genomics toolbox is the development of morpholino-based antisense gene inhibition for sequence-based 'knockdown' screening. This method offers the opportunity to examine the role of significant subsets of the vertebrate genome for specific gene function in vivo. The zebrafish embryo can rapidly provide critical information for drug target discovery purposes when examined with an emphasis on clinically-relevant biological processes. Finally, the advent of chemical genetics in zebrafish suggests that, in addition to the identification and understanding of drug targets and their biology, this system will be a powerful tool in the direct development of novel pharmaceuticals in the near future.

Original languageEnglish (US)
Pages (from-to)409-413
Number of pages5
JournalCurrent Pharmaceutical Biotechnology
Volume5
Issue number5
DOIs
StatePublished - Oct 2004
Externally publishedYes

Fingerprint

Zebrafish
Genomics
Research
Vertebrates
Genome
Biological Phenomena
Reverse Genetics
Morpholinos
Systems Biology
Drug Discovery
Invertebrates
Diptera
Pharmaceutical Preparations
Genes
Embryonic Structures
Animal Models

Keywords

  • Functional genomics
  • Gene discovery
  • Morpholino
  • Zebrafish

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Molecular Medicine
  • Biotechnology

Cite this

Zebrafish as a genomics research model. / Chen, Eleanor; Ekker, Stephen C.

In: Current Pharmaceutical Biotechnology, Vol. 5, No. 5, 10.2004, p. 409-413.

Research output: Contribution to journalArticle

@article{811f052828e74daebc1a12bae276e5e5,
title = "Zebrafish as a genomics research model",
abstract = "The zebrafish (Danio rerio) is a recent addition to the genomic scientists' repertoire of vertebrate animal model systems. Unlike simple invertebrates such as the fly or the nematode, this teleost maintains the biological and genomic complexity found in higher vertebrates. Furthermore, the zebrafish has many advantageous technical and genomic properties that open the door to experimental approaches not practical using more classical models. The zebrafish genome can be functionally accessed using both forward and reverse genetics based approaches. A notable recent addition to the zebrafish genomics toolbox is the development of morpholino-based antisense gene inhibition for sequence-based 'knockdown' screening. This method offers the opportunity to examine the role of significant subsets of the vertebrate genome for specific gene function in vivo. The zebrafish embryo can rapidly provide critical information for drug target discovery purposes when examined with an emphasis on clinically-relevant biological processes. Finally, the advent of chemical genetics in zebrafish suggests that, in addition to the identification and understanding of drug targets and their biology, this system will be a powerful tool in the direct development of novel pharmaceuticals in the near future.",
keywords = "Functional genomics, Gene discovery, Morpholino, Zebrafish",
author = "Eleanor Chen and Ekker, {Stephen C}",
year = "2004",
month = "10",
doi = "10.2174/1389201043376652",
language = "English (US)",
volume = "5",
pages = "409--413",
journal = "Current Pharmaceutical Biotechnology",
issn = "1389-2010",
publisher = "Bentham Science Publishers B.V.",
number = "5",

}

TY - JOUR

T1 - Zebrafish as a genomics research model

AU - Chen, Eleanor

AU - Ekker, Stephen C

PY - 2004/10

Y1 - 2004/10

N2 - The zebrafish (Danio rerio) is a recent addition to the genomic scientists' repertoire of vertebrate animal model systems. Unlike simple invertebrates such as the fly or the nematode, this teleost maintains the biological and genomic complexity found in higher vertebrates. Furthermore, the zebrafish has many advantageous technical and genomic properties that open the door to experimental approaches not practical using more classical models. The zebrafish genome can be functionally accessed using both forward and reverse genetics based approaches. A notable recent addition to the zebrafish genomics toolbox is the development of morpholino-based antisense gene inhibition for sequence-based 'knockdown' screening. This method offers the opportunity to examine the role of significant subsets of the vertebrate genome for specific gene function in vivo. The zebrafish embryo can rapidly provide critical information for drug target discovery purposes when examined with an emphasis on clinically-relevant biological processes. Finally, the advent of chemical genetics in zebrafish suggests that, in addition to the identification and understanding of drug targets and their biology, this system will be a powerful tool in the direct development of novel pharmaceuticals in the near future.

AB - The zebrafish (Danio rerio) is a recent addition to the genomic scientists' repertoire of vertebrate animal model systems. Unlike simple invertebrates such as the fly or the nematode, this teleost maintains the biological and genomic complexity found in higher vertebrates. Furthermore, the zebrafish has many advantageous technical and genomic properties that open the door to experimental approaches not practical using more classical models. The zebrafish genome can be functionally accessed using both forward and reverse genetics based approaches. A notable recent addition to the zebrafish genomics toolbox is the development of morpholino-based antisense gene inhibition for sequence-based 'knockdown' screening. This method offers the opportunity to examine the role of significant subsets of the vertebrate genome for specific gene function in vivo. The zebrafish embryo can rapidly provide critical information for drug target discovery purposes when examined with an emphasis on clinically-relevant biological processes. Finally, the advent of chemical genetics in zebrafish suggests that, in addition to the identification and understanding of drug targets and their biology, this system will be a powerful tool in the direct development of novel pharmaceuticals in the near future.

KW - Functional genomics

KW - Gene discovery

KW - Morpholino

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=4844220944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4844220944&partnerID=8YFLogxK

U2 - 10.2174/1389201043376652

DO - 10.2174/1389201043376652

M3 - Article

C2 - 15544488

AN - SCOPUS:4844220944

VL - 5

SP - 409

EP - 413

JO - Current Pharmaceutical Biotechnology

JF - Current Pharmaceutical Biotechnology

SN - 1389-2010

IS - 5

ER -