Wavelength and fluence effect on vascular damage with photodynamic therapy on skin

Maria M. Tsoukas, Salvador González, Thomas J Flotte, R. Rox Anderson, Margaret E. Sherwood, Nikiforos Kollias

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Normal skin phototoxicity is clinically predictable during photodynamic therapy with light at 690 and 458 nm wavelengths, in the first 5 h after intravenous bolus infusion of benzoporphyrin derivative mono-acid ring A. This study goal was to determine histologic milestones that lead to tissue necrosis with exposure to red (690nm) and blue (458nm) light. The threshold doses for skin necrosis on rabbits were equal at both wavelengths. Lower, equal to, and higher than threshold fluences were delivered in duplicates at hourly intervals, with 40% increments, at constant irradiance. Pathology specimens from irradiated and control sites, were collected at 0, 2, 7, 24, 48 h, and 2 wk after treatment and were paired to equivalent treated sites for clinical evaluation. Immediately after irradiation, at 690 and 458nm thresholds, light microscopy showed stasis and inflammatory infiltrate in the papillary dermis, respectively; electron microscopy demonstrated pericyte and endothelial cell damage - greater at 690 than 458nm. At day 1, vascular stasis in the dermis showed a steeper dose-response with red than blue light, and led to necrosis of skin appendages (day 1) and epidermis (days 1-2) at both wavelengths. Subthreshold fluences induced similar, but significantly milder (p <0.05) changes and epidermis recovered. Skin necrosis, at threshold fluences in photodynamic therapy with benzoporphyrin derivative mono-acid ring A, was primarily due to vascular compromise to a depth potentially reaching the subcutaneous muscle at 690nm, whereas at 458nm vascular damage was confined to upper dermis. This system facilitates selective destruction of skin vasculature, sparing normal epidermis.

Original languageEnglish (US)
Pages (from-to)303-308
Number of pages6
JournalJournal of Investigative Dermatology
Volume114
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Photodynamic therapy
Photochemotherapy
Blood Vessels
Skin
Necrosis
Wavelength
Dermis
Epidermis
Light
Dosimetry
Phototoxic Dermatitis
Derivatives
Pericytes
Acids
Endothelial cells
Pathology
Intravenous Infusions
Electron microscopy
Optical microscopy
Muscle

Keywords

  • Benzoporphyrin derivative monoacid ring A
  • Phototoxicity
  • Skin histology

ASJC Scopus subject areas

  • Dermatology

Cite this

Wavelength and fluence effect on vascular damage with photodynamic therapy on skin. / Tsoukas, Maria M.; González, Salvador; Flotte, Thomas J; Rox Anderson, R.; Sherwood, Margaret E.; Kollias, Nikiforos.

In: Journal of Investigative Dermatology, Vol. 114, No. 2, 2000, p. 303-308.

Research output: Contribution to journalArticle

Tsoukas, Maria M. ; González, Salvador ; Flotte, Thomas J ; Rox Anderson, R. ; Sherwood, Margaret E. ; Kollias, Nikiforos. / Wavelength and fluence effect on vascular damage with photodynamic therapy on skin. In: Journal of Investigative Dermatology. 2000 ; Vol. 114, No. 2. pp. 303-308.
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