@inbook{0cc7090facaa447b8bbdefb59e7e2227,
title = "Viral Gene Delivery in Chondrocytes",
abstract = "Viral gene transfer, known as transduction, is a powerful research tool for studying the biology of chondrocytes in novel ways and also a technology enabling the use of gene therapy for regenerating cartilage and treating diseases that affect cartilage, such as osteoarthritis. Adenovirus, retrovirus, lentivirus, and adeno-associated virus (AAV) are most commonly used to transduce chondrocytes. Although AAV is able to transduce chondrocytes in situ by intra-articular injection, chondrocytes are most commonly transduced in monolayer culture using the four vectors mentioned above. Protocols for achieving this are described, along with a discussion of the variables that can influence transduction efficiency.",
keywords = "AAV, Adenovirus, Cartilage, Lentivirus, Retrovirus",
author = "Nagelli, {Christopher V.} and Evans, {Christopher H.} and {De La Vega}, {Rodolfo E.}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2839-3_20",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "289--300",
booktitle = "Methods in Molecular Biology",
}