Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection

Surbhi Leekha; Cole L. Irish; Susan K. Schneider; Emily C. Fernholz; Mark J. Espy; Scott A. Cunningham; Robin Patel; Young J. Juhn; Bobbi Pritt; Thomas F. Smith; Priya Sampathkumar

doi:10.1016/j.diagmicrobio.2012.10.016

Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection

Surbhi Leekha, Cole L. Irish, Susan K. Schneider, Emily C. Fernholz, Mark J. Espy, Scott A. Cunningham, Robin Patel, Young J. Juhn, Bobbi Pritt, Thomas F. Smith, Priya Sampathkumar

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We evaluated a commercial multiplex polymerase chain reaction (PCR) assay in a cross-sectional study among 81 adult and pediatric outpatients-40 cases with upper respiratory infection symptoms and 41 asymptomatic controls-from February to April 2008. Two specimens (throat swab and nasal swab) from each participant were tested using the EraGen MultiCode-PLx Respiratory Virus Panel that detects 17 viral targets. Throat swabs were also tested for Group A Streptococcus (GAS) by PCR. Respiratory viruses were detected in 22/40 (55%) cases and in 3/41 (7%) controls (P < 0.001). GAS was detected in 10 (25%) cases; GAS and respiratory virus co-infection was found in 4 (10%). Agreement between nasal and throat swabs for viral detection was 69% in cases and 95% in controls. Of 22 cases with a detectable virus, 12 (54%) were picked up by only 1 (throat or nasal) specimen, and the detection rate was increased by combining results of nasal and throat swab testing.

Original language	English (US)
Pages (from-to)	169-173
Number of pages	5
Journal	Diagnostic Microbiology and Infectious Disease
Volume	75
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2012.10.016
State	Published - Feb 2013

Keywords

Group A streptococcus
Multiplex PCR
Nasal swab
Respiratory virus
Throat swab

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1016/j.diagmicrobio.2012.10.016

Cite this

Leekha, S., Irish, C. L., Schneider, S. K., Fernholz, E. C., Espy, M. J., Cunningham, S. A., Patel, R., Juhn, Y. J., Pritt, B., Smith, T. F., & Sampathkumar, P. (2013). Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection. Diagnostic Microbiology and Infectious Disease, 75(2), 169-173. https://doi.org/10.1016/j.diagmicrobio.2012.10.016

Leekha, S, Irish, CL, Schneider, SK, Fernholz, EC, Espy, MJ, Cunningham, SA, Patel, R , Juhn, YJ, Pritt, B, Smith, TF & Sampathkumar, P 2013, 'Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection', Diagnostic Microbiology and Infectious Disease, vol. 75, no. 2, pp. 169-173. https://doi.org/10.1016/j.diagmicrobio.2012.10.016

@article{d337b16141174271ae76c69d92cb8868,

title = "Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection",

abstract = "We evaluated a commercial multiplex polymerase chain reaction (PCR) assay in a cross-sectional study among 81 adult and pediatric outpatients-40 cases with upper respiratory infection symptoms and 41 asymptomatic controls-from February to April 2008. Two specimens (throat swab and nasal swab) from each participant were tested using the EraGen MultiCode-PLx Respiratory Virus Panel that detects 17 viral targets. Throat swabs were also tested for Group A Streptococcus (GAS) by PCR. Respiratory viruses were detected in 22/40 (55%) cases and in 3/41 (7%) controls (P < 0.001). GAS was detected in 10 (25%) cases; GAS and respiratory virus co-infection was found in 4 (10%). Agreement between nasal and throat swabs for viral detection was 69% in cases and 95% in controls. Of 22 cases with a detectable virus, 12 (54%) were picked up by only 1 (throat or nasal) specimen, and the detection rate was increased by combining results of nasal and throat swab testing.",

keywords = "Group A streptococcus, Multiplex PCR, Nasal swab, Respiratory virus, Throat swab",

author = "Surbhi Leekha and Irish, {Cole L.} and Schneider, {Susan K.} and Fernholz, {Emily C.} and Espy, {Mark J.} and Cunningham, {Scott A.} and Robin Patel and Juhn, {Young J.} and Bobbi Pritt and Smith, {Thomas F.} and Priya Sampathkumar",

year = "2013",

month = feb,

doi = "10.1016/j.diagmicrobio.2012.10.016",

language = "English (US)",

volume = "75",

pages = "169--173",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection

AU - Leekha, Surbhi

AU - Irish, Cole L.

AU - Schneider, Susan K.

AU - Fernholz, Emily C.

AU - Espy, Mark J.

AU - Cunningham, Scott A.

AU - Patel, Robin

AU - Juhn, Young J.

AU - Pritt, Bobbi

AU - Smith, Thomas F.

AU - Sampathkumar, Priya

PY - 2013/2

Y1 - 2013/2

N2 - We evaluated a commercial multiplex polymerase chain reaction (PCR) assay in a cross-sectional study among 81 adult and pediatric outpatients-40 cases with upper respiratory infection symptoms and 41 asymptomatic controls-from February to April 2008. Two specimens (throat swab and nasal swab) from each participant were tested using the EraGen MultiCode-PLx Respiratory Virus Panel that detects 17 viral targets. Throat swabs were also tested for Group A Streptococcus (GAS) by PCR. Respiratory viruses were detected in 22/40 (55%) cases and in 3/41 (7%) controls (P < 0.001). GAS was detected in 10 (25%) cases; GAS and respiratory virus co-infection was found in 4 (10%). Agreement between nasal and throat swabs for viral detection was 69% in cases and 95% in controls. Of 22 cases with a detectable virus, 12 (54%) were picked up by only 1 (throat or nasal) specimen, and the detection rate was increased by combining results of nasal and throat swab testing.

AB - We evaluated a commercial multiplex polymerase chain reaction (PCR) assay in a cross-sectional study among 81 adult and pediatric outpatients-40 cases with upper respiratory infection symptoms and 41 asymptomatic controls-from February to April 2008. Two specimens (throat swab and nasal swab) from each participant were tested using the EraGen MultiCode-PLx Respiratory Virus Panel that detects 17 viral targets. Throat swabs were also tested for Group A Streptococcus (GAS) by PCR. Respiratory viruses were detected in 22/40 (55%) cases and in 3/41 (7%) controls (P < 0.001). GAS was detected in 10 (25%) cases; GAS and respiratory virus co-infection was found in 4 (10%). Agreement between nasal and throat swabs for viral detection was 69% in cases and 95% in controls. Of 22 cases with a detectable virus, 12 (54%) were picked up by only 1 (throat or nasal) specimen, and the detection rate was increased by combining results of nasal and throat swab testing.

KW - Group A streptococcus

KW - Multiplex PCR

KW - Nasal swab

KW - Respiratory virus

KW - Throat swab

UR - http://www.scopus.com/inward/record.url?scp=84872491029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872491029&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2012.10.016

DO - 10.1016/j.diagmicrobio.2012.10.016

M3 - Article

C2 - 23182565

AN - SCOPUS:84872491029

SN - 0732-8893

VL - 75

SP - 169

EP - 173

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 2

ER -

Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this