Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection

Surbhi Leekha, Cole L. Irish, Susan K. Schneider, Emily C. Fernholz, Mark J. Espy, Scott A. Cunningham, Robin Patel, Young J. Juhn, Bobbi Pritt, Thomas F. Smith, Priya Sampathkumar

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We evaluated a commercial multiplex polymerase chain reaction (PCR) assay in a cross-sectional study among 81 adult and pediatric outpatients-40 cases with upper respiratory infection symptoms and 41 asymptomatic controls-from February to April 2008. Two specimens (throat swab and nasal swab) from each participant were tested using the EraGen MultiCode-PLx Respiratory Virus Panel that detects 17 viral targets. Throat swabs were also tested for Group A Streptococcus (GAS) by PCR. Respiratory viruses were detected in 22/40 (55%) cases and in 3/41 (7%) controls (P < 0.001). GAS was detected in 10 (25%) cases; GAS and respiratory virus co-infection was found in 4 (10%). Agreement between nasal and throat swabs for viral detection was 69% in cases and 95% in controls. Of 22 cases with a detectable virus, 12 (54%) were picked up by only 1 (throat or nasal) specimen, and the detection rate was increased by combining results of nasal and throat swab testing.

Original languageEnglish (US)
Pages (from-to)169-173
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume75
Issue number2
DOIs
StatePublished - Feb 2013

Keywords

  • Group A streptococcus
  • Multiplex PCR
  • Nasal swab
  • Respiratory virus
  • Throat swab

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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