Validation of 3-deoxy-3-fluoro-D-glucose as a glucose transport analogue in rat heart

Four aspects of the behavior of 3-deoxy-3-fluoro-D-glucose (D-3FDG) were studied. 1) The distribution of label in rat tissues after intravenous administration of [¹⁸F]D-3FDG was compared with that seen with labeled 3-deoxy-3-fluoro-L-glucose (L-3FDG). Results were consistent with a larger volume of distribution for the physiological D-isomer coupled with some degree of reabsorption by the kidneys. L-3FDG, but not its D-isomer, was excluded from the brain. 2) D-3FDG competitively inhibited uptake of glucose by isolated perfused rat hearts. The inhibition constant was 12.8 ± 1.6 mM compared with 6.1 ± 1.1 mM for 3-O-methyl-D-glucose. 3) Residue curves obtained after bolus administration of [¹⁸F]D-3FDG to isolated hearts indicated phosphorylation of the tracer at a lower rate than for 2-deoxy-2-fluoro-D-glucose but with subsequent dephosphorylation at a faster rate. 4) Chromatographic analysis of ¹⁸F remaining in tissues after administration of [¹⁸F]D-3FDG revealed in addition to free D-3FDG three other peaks. These disappeared after treatment with alkaline phosphatase and were thus assigned as phosphates. The principal metabolite had the same retention time as D-3FDG-6-phosphate prepared with hexokinase. No phosphorylated metabolites were detected in blood. D-3FDG labeled with ¹⁸F may be a useful tracer in studies of glucose transport and metabolism.