Validation of 3-deoxy-3-fluoro-D-glucose as a glucose transport analogue in rat heart.

J. R. Halama, S. J. Gatley, Timothy R DeGrado, D. R. Bernstein, C. K. Ng, J. E. Holden

Research output: Contribution to journalArticle

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Abstract

Four aspects of the behavior of 3-deoxy-3-fluoro-D-glucose (D-3FDG) were studied. The distribution of label in rat tissues after intravenous administration of [18F]D-3FDG was compared with that seen with labeled 3-deoxy-3-fluoro-L-glucose (L-3FDG). Results were consistent with a larger volume of distribution for the physiological D-isomer coupled with some degree of reabsorption by the kidneys. L-3FDG, but not its D-isomer, was excluded from the brain. D-3FDG competitively inhibited uptake of glucose by isolated perfused rat hearts. The inhibition constant was 12.8 +/- 1.6 mM compared with 6.1 +/- 1.1 mM for 3-O-methyl-D-glucose. Residue curves obtained after bolus administration of [18F]D-3FDG to isolated hearts indicated phosphorylation of the tracer at a lower rate than for 2-deoxy-2-fluoro-D-glucose but with subsequent dephosphorylation at a faster rate. Chromatographic analysis of 18F remaining in tissues after administration of [18F]D-3FDG revealed in addition to free D-3FDG three other peaks. These disappeared after treatment with alkaline phosphatase and were thus assigned as phosphates. The principal metabolite had the same retention time as D-3FDG-6-phosphate prepared with hexokinase. No phosphorylated metabolites were detected in blood. D-3FDG labeled with 18F may be a useful tracer in studies of glucose transport and metabolism.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume247
Issue number5 Pt 2
StatePublished - Nov 1984
Externally publishedYes

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Glucose
3-deoxy-3-fluoro-D-glucose
3-O-Methylglucose
Glucose-6-Phosphate
Hexokinase
Fluorodeoxyglucose F18
Intravenous Administration
Alkaline Phosphatase
Chromatography
Phosphates
Phosphorylation
Kidney
Brain

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Halama, J. R., Gatley, S. J., DeGrado, T. R., Bernstein, D. R., Ng, C. K., & Holden, J. E. (1984). Validation of 3-deoxy-3-fluoro-D-glucose as a glucose transport analogue in rat heart. The American journal of physiology, 247(5 Pt 2).

Validation of 3-deoxy-3-fluoro-D-glucose as a glucose transport analogue in rat heart. / Halama, J. R.; Gatley, S. J.; DeGrado, Timothy R; Bernstein, D. R.; Ng, C. K.; Holden, J. E.

In: The American journal of physiology, Vol. 247, No. 5 Pt 2, 11.1984.

Research output: Contribution to journalArticle

Halama, JR, Gatley, SJ, DeGrado, TR, Bernstein, DR, Ng, CK & Holden, JE 1984, 'Validation of 3-deoxy-3-fluoro-D-glucose as a glucose transport analogue in rat heart.', The American journal of physiology, vol. 247, no. 5 Pt 2.
Halama, J. R. ; Gatley, S. J. ; DeGrado, Timothy R ; Bernstein, D. R. ; Ng, C. K. ; Holden, J. E. / Validation of 3-deoxy-3-fluoro-D-glucose as a glucose transport analogue in rat heart. In: The American journal of physiology. 1984 ; Vol. 247, No. 5 Pt 2.
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