Validation and clinicopathologic associations of a urine-based bladder cancer biomarker signature

Ge Zhang, Evan Gomes-Giacoia, Yunfeng Dai, Adrienne Lawton, Makito Miyake, Hideki Furuya, Steven Goodison, Charles J. Rosser

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

BACKGROUND: To validate the expression of a urine-based bladder cancer associated diagnostic signature comprised of 10 targets; ANG, CA9, MMP9, MMP10, SERPINA1, APOE, SDC1, VEGFA, SERPINE1 and IL8 in bladder tumor tissues.

METHODS: Immunohistochemical analyses were performed on tumor specimens from 213 bladder cancer patients (transitional cell carcinoma only) and 74 controls. Staining patterns were digitally captured and quantitated (Aperio, Vista, CA), and expression was correlated with tumor stage, tumor grade and outcome measures.

RESULTS: We revealed a positive association of 9 of the 10 proteins (excluding VEGF) in bladder cancer. Relative to control cases, a reduction in SDC1 and overexpression of MMP9, MMP10, SERPINE1, IL8, APOE, SERPINA1, ANG were associated with high stage bladder cancer. Reduced VEGF and increased SERPINA1 were associated with high-grade bladder cancer. Disease-specific survival was significantly reduced in tumors with high expression of SERPINE1 and/or IL8.

CONCLUSIONS: These findings confirm that the proteins in a urine-based diagnostic signature are aberrantly expressed in bladder tumor tissues, and support the potential additional utility of selected biomarkers for the clinicopathological evaluation of excised tissue or biopsy material.

VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_200.

Original languageEnglish (US)
Number of pages1
JournalDiagnostic Pathology
Volume9
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Tumor Biomarkers
Urinary Bladder Neoplasms
Urine
Interleukin-8
Apolipoproteins E
Vascular Endothelial Growth Factor A
Neoplasms
Transitional Cell Carcinoma
Proteins
Biomarkers
Outcome Assessment (Health Care)
Staining and Labeling
Biopsy
Survival

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Zhang, G., Gomes-Giacoia, E., Dai, Y., Lawton, A., Miyake, M., Furuya, H., ... Rosser, C. J. (2014). Validation and clinicopathologic associations of a urine-based bladder cancer biomarker signature. Diagnostic Pathology, 9. https://doi.org/10.1186/s13000-014-0200-1

Validation and clinicopathologic associations of a urine-based bladder cancer biomarker signature. / Zhang, Ge; Gomes-Giacoia, Evan; Dai, Yunfeng; Lawton, Adrienne; Miyake, Makito; Furuya, Hideki; Goodison, Steven; Rosser, Charles J.

In: Diagnostic Pathology, Vol. 9, 01.01.2014.

Research output: Contribution to journalArticle

Zhang, Ge ; Gomes-Giacoia, Evan ; Dai, Yunfeng ; Lawton, Adrienne ; Miyake, Makito ; Furuya, Hideki ; Goodison, Steven ; Rosser, Charles J. / Validation and clinicopathologic associations of a urine-based bladder cancer biomarker signature. In: Diagnostic Pathology. 2014 ; Vol. 9.
@article{b1f5342b28d24e10923fc764c6890741,
title = "Validation and clinicopathologic associations of a urine-based bladder cancer biomarker signature",
abstract = "BACKGROUND: To validate the expression of a urine-based bladder cancer associated diagnostic signature comprised of 10 targets; ANG, CA9, MMP9, MMP10, SERPINA1, APOE, SDC1, VEGFA, SERPINE1 and IL8 in bladder tumor tissues.METHODS: Immunohistochemical analyses were performed on tumor specimens from 213 bladder cancer patients (transitional cell carcinoma only) and 74 controls. Staining patterns were digitally captured and quantitated (Aperio, Vista, CA), and expression was correlated with tumor stage, tumor grade and outcome measures.RESULTS: We revealed a positive association of 9 of the 10 proteins (excluding VEGF) in bladder cancer. Relative to control cases, a reduction in SDC1 and overexpression of MMP9, MMP10, SERPINE1, IL8, APOE, SERPINA1, ANG were associated with high stage bladder cancer. Reduced VEGF and increased SERPINA1 were associated with high-grade bladder cancer. Disease-specific survival was significantly reduced in tumors with high expression of SERPINE1 and/or IL8.CONCLUSIONS: These findings confirm that the proteins in a urine-based diagnostic signature are aberrantly expressed in bladder tumor tissues, and support the potential additional utility of selected biomarkers for the clinicopathological evaluation of excised tissue or biopsy material.VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_200.",
author = "Ge Zhang and Evan Gomes-Giacoia and Yunfeng Dai and Adrienne Lawton and Makito Miyake and Hideki Furuya and Steven Goodison and Rosser, {Charles J.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1186/s13000-014-0200-1",
language = "English (US)",
volume = "9",
journal = "Diagnostic Pathology",
issn = "1746-1596",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Validation and clinicopathologic associations of a urine-based bladder cancer biomarker signature

AU - Zhang, Ge

AU - Gomes-Giacoia, Evan

AU - Dai, Yunfeng

AU - Lawton, Adrienne

AU - Miyake, Makito

AU - Furuya, Hideki

AU - Goodison, Steven

AU - Rosser, Charles J.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - BACKGROUND: To validate the expression of a urine-based bladder cancer associated diagnostic signature comprised of 10 targets; ANG, CA9, MMP9, MMP10, SERPINA1, APOE, SDC1, VEGFA, SERPINE1 and IL8 in bladder tumor tissues.METHODS: Immunohistochemical analyses were performed on tumor specimens from 213 bladder cancer patients (transitional cell carcinoma only) and 74 controls. Staining patterns were digitally captured and quantitated (Aperio, Vista, CA), and expression was correlated with tumor stage, tumor grade and outcome measures.RESULTS: We revealed a positive association of 9 of the 10 proteins (excluding VEGF) in bladder cancer. Relative to control cases, a reduction in SDC1 and overexpression of MMP9, MMP10, SERPINE1, IL8, APOE, SERPINA1, ANG were associated with high stage bladder cancer. Reduced VEGF and increased SERPINA1 were associated with high-grade bladder cancer. Disease-specific survival was significantly reduced in tumors with high expression of SERPINE1 and/or IL8.CONCLUSIONS: These findings confirm that the proteins in a urine-based diagnostic signature are aberrantly expressed in bladder tumor tissues, and support the potential additional utility of selected biomarkers for the clinicopathological evaluation of excised tissue or biopsy material.VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_200.

AB - BACKGROUND: To validate the expression of a urine-based bladder cancer associated diagnostic signature comprised of 10 targets; ANG, CA9, MMP9, MMP10, SERPINA1, APOE, SDC1, VEGFA, SERPINE1 and IL8 in bladder tumor tissues.METHODS: Immunohistochemical analyses were performed on tumor specimens from 213 bladder cancer patients (transitional cell carcinoma only) and 74 controls. Staining patterns were digitally captured and quantitated (Aperio, Vista, CA), and expression was correlated with tumor stage, tumor grade and outcome measures.RESULTS: We revealed a positive association of 9 of the 10 proteins (excluding VEGF) in bladder cancer. Relative to control cases, a reduction in SDC1 and overexpression of MMP9, MMP10, SERPINE1, IL8, APOE, SERPINA1, ANG were associated with high stage bladder cancer. Reduced VEGF and increased SERPINA1 were associated with high-grade bladder cancer. Disease-specific survival was significantly reduced in tumors with high expression of SERPINE1 and/or IL8.CONCLUSIONS: These findings confirm that the proteins in a urine-based diagnostic signature are aberrantly expressed in bladder tumor tissues, and support the potential additional utility of selected biomarkers for the clinicopathological evaluation of excised tissue or biopsy material.VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_200.

UR - http://www.scopus.com/inward/record.url?scp=84965188238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84965188238&partnerID=8YFLogxK

U2 - 10.1186/s13000-014-0200-1

DO - 10.1186/s13000-014-0200-1

M3 - Article

C2 - 25387487

AN - SCOPUS:84965188238

VL - 9

JO - Diagnostic Pathology

JF - Diagnostic Pathology

SN - 1746-1596

ER -