Vaccination with allogeneic dendritic cells fused to carcinoma cells induces antitumor immunity in MUC1 transgenic mice

Yasuhiro Tanaka, Shigeo Koido, Dongshu Chen, Sandra J Gendler, Donald Kufe, Jianlin Gong

Research output: Contribution to journalArticle

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Abstract

Fusions of autologous tumor cells with allogeneic dendritic cells (DC) represent an approach for the induction of antitumor immunity. In the present studies, we investigated the antitumor effects of vaccinating MUC1-transgenic (MUC1.Tg) mice with MC38/MUC1 carcinoma cells fused to allogeneic DC from BALB/c mice (allo-DC, H-2d) or syngeneic DC from C57BL/6 mice (syn-DC, H-2b). Both allo and syn fusion cells (FC/MUC1) expressed MHC class II, costimulatory molecules, and the MUC1 antigen. Alloo-FC/MUC1 exhibited dual expression of MHC class I haplotypes (H-2d/H-2b) and MUC1 antigen. By contrast, only H-2b and MUC1 antigen were expressed by syn-FC/MUC1. CTLs from MUC1.Tg mice immunized with allo- or syn-FC/MUC1 fusion cells lysed MC38/MUC1 targets. Moreover, immunization with allo- or syn-FC/MUC1 was effective in eliminating established MUC1-positive pulmonary metastases in MUC1.Tg mice. These results indicate that immunization of MUC1.Tg mice with syn- or allo-FC/MUC1 is effective in reversing immunologic unresponsiveness to MUC1 antigen and inducing immunity against MUC1-positive tumors. The findings in the present study have broader clinical implications for fusion cell vaccines.

Original languageEnglish (US)
Pages (from-to)192-200
Number of pages9
JournalClinical Immunology
Volume101
Issue number2
DOIs
StatePublished - 2001

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Dendritic Cells
Transgenic Mice
Immunity
Vaccination
Cell Fusion
Carcinoma
Antigens
Immunization
Inbred C57BL Mouse
Haplotypes
Neoplasms
Vaccines
Neoplasm Metastasis
Lung

Keywords

  • Allogeneic and syngeneic DC fusions
  • Anti-tumor immunity
  • Fusion cell vaccines
  • Reversal of tolerance to MUC1

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Vaccination with allogeneic dendritic cells fused to carcinoma cells induces antitumor immunity in MUC1 transgenic mice. / Tanaka, Yasuhiro; Koido, Shigeo; Chen, Dongshu; Gendler, Sandra J; Kufe, Donald; Gong, Jianlin.

In: Clinical Immunology, Vol. 101, No. 2, 2001, p. 192-200.

Research output: Contribution to journalArticle

Tanaka, Yasuhiro ; Koido, Shigeo ; Chen, Dongshu ; Gendler, Sandra J ; Kufe, Donald ; Gong, Jianlin. / Vaccination with allogeneic dendritic cells fused to carcinoma cells induces antitumor immunity in MUC1 transgenic mice. In: Clinical Immunology. 2001 ; Vol. 101, No. 2. pp. 192-200.
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