Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma

Andras Khoor, Jeffrey A. Whitsett, Mildred T. Stahlman, Sandra J. Olson, Philip T. Cagle

Research output: Contribution to journalArticle

132 Scopus citations

Abstract

Differentiation of malignant mesothelioma from adenocarcinoma, particularly from a lung primary, remains a difficult diagnostic problem. Surfactant protein B precursor (pro-SP-B) and thyroid transcription factor 1 (TTF-1) are expressed selectively in the normal respiratory epithelium and in adenocarcinomas of the lung. In this study, we evaluated the utility of pro- SP-B and TTF-1 in distinguishing pulmonary adenocarcinomas and malignant mesotheliomas. Immunoreactivity for pro-SP-B and TTF-1 was examined in paraffin sections of 370 primary lung carcinomas (208 adenocarcinomas, 101 squamous cell carcinomas, and 61 large cell carcinomas) and 95 malignant mesotheliomas, using a pro-SP-B antiserum and a monoclonal TTF-1 antibody with a biotin-streptavidin detection system. Immunostaining for pro-SP-B was detected in 57% of adenocarcinomas, and 20% of large cell carcinomas. Immunoreactivity for TTF-1 was shown in 76% of adenocarcinomas and 26% of large cell carcinomas. Malignant mesotheliomas and squamous cell carcinomas did not stain with either antibody. The expression of pro-SP-B and TTF-1 in adenocarcinomas of the lung but not in malignant mesotheliomas shows that pro-SP-B and TTF-1 staining is useful in differentiating these neoplasms.

Original languageEnglish (US)
Pages (from-to)695-700
Number of pages6
JournalHuman Pathology
Volume30
Issue number6
DOIs
StatePublished - Jun 1999

Keywords

  • Adenocarcinoma
  • Lung
  • Malignant mesothelioma
  • TTF-1
  • proSP-B

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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