Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma

Andras Khoor, Jeffrey A. Whitsett, Mildred T. Stahlman, Sandra J. Olson, Philip T. Cagle

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Differentiation of malignant mesothelioma from adenocarcinoma, particularly from a lung primary, remains a difficult diagnostic problem. Surfactant protein B precursor (pro-SP-B) and thyroid transcription factor 1 (TTF-1) are expressed selectively in the normal respiratory epithelium and in adenocarcinomas of the lung. In this study, we evaluated the utility of pro- SP-B and TTF-1 in distinguishing pulmonary adenocarcinomas and malignant mesotheliomas. Immunoreactivity for pro-SP-B and TTF-1 was examined in paraffin sections of 370 primary lung carcinomas (208 adenocarcinomas, 101 squamous cell carcinomas, and 61 large cell carcinomas) and 95 malignant mesotheliomas, using a pro-SP-B antiserum and a monoclonal TTF-1 antibody with a biotin-streptavidin detection system. Immunostaining for pro-SP-B was detected in 57% of adenocarcinomas, and 20% of large cell carcinomas. Immunoreactivity for TTF-1 was shown in 76% of adenocarcinomas and 26% of large cell carcinomas. Malignant mesotheliomas and squamous cell carcinomas did not stain with either antibody. The expression of pro-SP-B and TTF-1 in adenocarcinomas of the lung but not in malignant mesotheliomas shows that pro-SP-B and TTF-1 staining is useful in differentiating these neoplasms.

Original languageEnglish (US)
Pages (from-to)695-700
Number of pages6
JournalHuman Pathology
Volume30
Issue number6
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Protein Precursors
Surface-Active Agents
Large Cell Carcinoma
Adenocarcinoma
Squamous Cell Carcinoma
Respiratory Mucosa
Lung
Streptavidin
Antibodies
Biotin
Adenocarcinoma of lung
thyroid nuclear factor 1
Malignant Mesothelioma
IgA receptor
Paraffin
Immune Sera
Coloring Agents
Staining and Labeling
Carcinoma
Neoplasms

Keywords

  • Adenocarcinoma
  • Lung
  • Malignant mesothelioma
  • proSP-B
  • TTF-1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma. / Khoor, Andras; Whitsett, Jeffrey A.; Stahlman, Mildred T.; Olson, Sandra J.; Cagle, Philip T.

In: Human Pathology, Vol. 30, No. 6, 1999, p. 695-700.

Research output: Contribution to journalArticle

Khoor, Andras ; Whitsett, Jeffrey A. ; Stahlman, Mildred T. ; Olson, Sandra J. ; Cagle, Philip T. / Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma. In: Human Pathology. 1999 ; Vol. 30, No. 6. pp. 695-700.
@article{25426b0fc5e94bf4aa32794c04e55538,
title = "Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma",
abstract = "Differentiation of malignant mesothelioma from adenocarcinoma, particularly from a lung primary, remains a difficult diagnostic problem. Surfactant protein B precursor (pro-SP-B) and thyroid transcription factor 1 (TTF-1) are expressed selectively in the normal respiratory epithelium and in adenocarcinomas of the lung. In this study, we evaluated the utility of pro- SP-B and TTF-1 in distinguishing pulmonary adenocarcinomas and malignant mesotheliomas. Immunoreactivity for pro-SP-B and TTF-1 was examined in paraffin sections of 370 primary lung carcinomas (208 adenocarcinomas, 101 squamous cell carcinomas, and 61 large cell carcinomas) and 95 malignant mesotheliomas, using a pro-SP-B antiserum and a monoclonal TTF-1 antibody with a biotin-streptavidin detection system. Immunostaining for pro-SP-B was detected in 57{\%} of adenocarcinomas, and 20{\%} of large cell carcinomas. Immunoreactivity for TTF-1 was shown in 76{\%} of adenocarcinomas and 26{\%} of large cell carcinomas. Malignant mesotheliomas and squamous cell carcinomas did not stain with either antibody. The expression of pro-SP-B and TTF-1 in adenocarcinomas of the lung but not in malignant mesotheliomas shows that pro-SP-B and TTF-1 staining is useful in differentiating these neoplasms.",
keywords = "Adenocarcinoma, Lung, Malignant mesothelioma, proSP-B, TTF-1",
author = "Andras Khoor and Whitsett, {Jeffrey A.} and Stahlman, {Mildred T.} and Olson, {Sandra J.} and Cagle, {Philip T.}",
year = "1999",
doi = "10.1016/S0046-8177(99)90096-5",
language = "English (US)",
volume = "30",
pages = "695--700",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "6",

}

TY - JOUR

T1 - Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma

AU - Khoor, Andras

AU - Whitsett, Jeffrey A.

AU - Stahlman, Mildred T.

AU - Olson, Sandra J.

AU - Cagle, Philip T.

PY - 1999

Y1 - 1999

N2 - Differentiation of malignant mesothelioma from adenocarcinoma, particularly from a lung primary, remains a difficult diagnostic problem. Surfactant protein B precursor (pro-SP-B) and thyroid transcription factor 1 (TTF-1) are expressed selectively in the normal respiratory epithelium and in adenocarcinomas of the lung. In this study, we evaluated the utility of pro- SP-B and TTF-1 in distinguishing pulmonary adenocarcinomas and malignant mesotheliomas. Immunoreactivity for pro-SP-B and TTF-1 was examined in paraffin sections of 370 primary lung carcinomas (208 adenocarcinomas, 101 squamous cell carcinomas, and 61 large cell carcinomas) and 95 malignant mesotheliomas, using a pro-SP-B antiserum and a monoclonal TTF-1 antibody with a biotin-streptavidin detection system. Immunostaining for pro-SP-B was detected in 57% of adenocarcinomas, and 20% of large cell carcinomas. Immunoreactivity for TTF-1 was shown in 76% of adenocarcinomas and 26% of large cell carcinomas. Malignant mesotheliomas and squamous cell carcinomas did not stain with either antibody. The expression of pro-SP-B and TTF-1 in adenocarcinomas of the lung but not in malignant mesotheliomas shows that pro-SP-B and TTF-1 staining is useful in differentiating these neoplasms.

AB - Differentiation of malignant mesothelioma from adenocarcinoma, particularly from a lung primary, remains a difficult diagnostic problem. Surfactant protein B precursor (pro-SP-B) and thyroid transcription factor 1 (TTF-1) are expressed selectively in the normal respiratory epithelium and in adenocarcinomas of the lung. In this study, we evaluated the utility of pro- SP-B and TTF-1 in distinguishing pulmonary adenocarcinomas and malignant mesotheliomas. Immunoreactivity for pro-SP-B and TTF-1 was examined in paraffin sections of 370 primary lung carcinomas (208 adenocarcinomas, 101 squamous cell carcinomas, and 61 large cell carcinomas) and 95 malignant mesotheliomas, using a pro-SP-B antiserum and a monoclonal TTF-1 antibody with a biotin-streptavidin detection system. Immunostaining for pro-SP-B was detected in 57% of adenocarcinomas, and 20% of large cell carcinomas. Immunoreactivity for TTF-1 was shown in 76% of adenocarcinomas and 26% of large cell carcinomas. Malignant mesotheliomas and squamous cell carcinomas did not stain with either antibody. The expression of pro-SP-B and TTF-1 in adenocarcinomas of the lung but not in malignant mesotheliomas shows that pro-SP-B and TTF-1 staining is useful in differentiating these neoplasms.

KW - Adenocarcinoma

KW - Lung

KW - Malignant mesothelioma

KW - proSP-B

KW - TTF-1

UR - http://www.scopus.com/inward/record.url?scp=0032997034&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032997034&partnerID=8YFLogxK

U2 - 10.1016/S0046-8177(99)90096-5

DO - 10.1016/S0046-8177(99)90096-5

M3 - Article

C2 - 10374779

AN - SCOPUS:0032997034

VL - 30

SP - 695

EP - 700

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 6

ER -