Using engineered endonucleases to create knockout and knockin zebrafish models

Victoria M. Bedell; Stephen C. Ekker

Using engineered endonucleases to create knockout and knockin zebrafish models

Biochemistry and Molecular Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Over the last few years, the technology to create targeted knockout and knockin zebrafish animals has exploded. We have gained the ability to create targeted knockouts through the use of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR associated system (CRISPR/Cas). Furthermore, using the high-efficiency TALEN system, we were able to create knockin zebrafish using a single-stranded DNA (ssDNA) protocol described here. Through the use of these technologies, the zebrafish has become a valuable vertebrate model and an excellent bridge between the invertebrate and mammalian model systems for the study of human disease.

Original language	English (US)
Title of host publication	Chromosomal Mutagenesis
Subtitle of host publication	Second Edition
Publisher	Springer Fachmedien
Pages	291-305
Number of pages	15
ISBN (Electronic)	9781493918621
ISBN (Print)	9781493918614
State	Published - Nov 19 2014

Keywords

Genome engineering
HDR
Homology directed repair
TALEN
Zebrafish

ASJC Scopus subject areas

General Medicine
General Biochemistry, Genetics and Molecular Biology

Cite this

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AB - Over the last few years, the technology to create targeted knockout and knockin zebrafish animals has exploded. We have gained the ability to create targeted knockouts through the use of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR associated system (CRISPR/Cas). Furthermore, using the high-efficiency TALEN system, we were able to create knockin zebrafish using a single-stranded DNA (ssDNA) protocol described here. Through the use of these technologies, the zebrafish has become a valuable vertebrate model and an excellent bridge between the invertebrate and mammalian model systems for the study of human disease.

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Using engineered endonucleases to create knockout and knockin zebrafish models

Abstract

Keywords

ASJC Scopus subject areas

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