Using engineered endonucleases to create knockout and knockin zebrafish models

Victoria M. Bedell, Stephen C Ekker

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Over the last few years, the technology to create targeted knockout and knockin zebrafish animals has exploded. We have gained the ability to create targeted knockouts through the use of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR associated system (CRISPR/Cas). Furthermore, using the high-efficiency TALEN system, we were able to create knockin zebrafish using a single-stranded DNA (ssDNA) protocol described here. Through the use of these technologies, the zebrafish has become a valuable vertebrate model and an excellent bridge between the invertebrate and mammalian model systems for the study of human disease.

Original languageEnglish (US)
Title of host publicationChromosomal Mutagenesis: Second Edition
PublisherSpringer Fachmedien
Pages291-305
Number of pages15
ISBN (Print)9781493918621, 9781493918614
StatePublished - Nov 19 2014

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Endonucleases
Zebrafish
Transcription
Single-Stranded DNA
Technology
Zinc
Animals
Zinc Fingers
Invertebrates
Vertebrates
Transcription Activator-Like Effector Nucleases

Keywords

  • Genome engineering
  • HDR
  • Homology directed repair
  • TALEN
  • Zebrafish

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bedell, V. M., & Ekker, S. C. (2014). Using engineered endonucleases to create knockout and knockin zebrafish models. In Chromosomal Mutagenesis: Second Edition (pp. 291-305). Springer Fachmedien.

Using engineered endonucleases to create knockout and knockin zebrafish models. / Bedell, Victoria M.; Ekker, Stephen C.

Chromosomal Mutagenesis: Second Edition. Springer Fachmedien, 2014. p. 291-305.

Research output: Chapter in Book/Report/Conference proceedingChapter

Bedell, VM & Ekker, SC 2014, Using engineered endonucleases to create knockout and knockin zebrafish models. in Chromosomal Mutagenesis: Second Edition. Springer Fachmedien, pp. 291-305.
Bedell VM, Ekker SC. Using engineered endonucleases to create knockout and knockin zebrafish models. In Chromosomal Mutagenesis: Second Edition. Springer Fachmedien. 2014. p. 291-305
Bedell, Victoria M. ; Ekker, Stephen C. / Using engineered endonucleases to create knockout and knockin zebrafish models. Chromosomal Mutagenesis: Second Edition. Springer Fachmedien, 2014. pp. 291-305
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