Aim: Microchimerism following canine in utero hematopoietic stem cell transplantation (IUHSCT) development of T-cell dosing regimens. Objective: To investigate the use of anti-T-cell antibodies for cell dosing of the donor graft in a canine model of IUHSCT. Study Design: Canine IUHSCT was performed by ultrasound-guided intraperitoneal injection in days 35-38 of fetal canines with CD34+ cells at doses of 4.5 × 108 to 1.3 × 109 cells/kg and T cells (CD3+ CD5+) at doses of 8 × 106 to 8.8 × 108 cells/kg. Postnatal studies included tissue histology and polymerase chain reaction-based chimerism analysis. Results: Term survival was 86-100%. Microchimerism (0-2%) was detected in five of eight recipients in multiple tissues. Histopathology revealed no evidence of graft-versus-host disease (GVHD). Conclusion: Canine IUHSCT is a useful model to investigate the role of donor T cells in engraftment and GVHD. IUHSCT at early gestational ages with high doses of donor T cells in the graft yields microchimerism in multiple tissues without GVHD.
- Bone marrow transplantation
- Canine model
- Hematopoietic stem cell
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
- Radiology Nuclear Medicine and imaging
- Obstetrics and Gynecology