Use of T-cell antibodies for donor dosaging in a canine model of in utero hematopoietic stem cell transplantation

Scott M. Petersen; Mariya Gendelman; Kathleen M. Murphy; Michael Torbenson; Richard J. Jones; Janyne E. Althaus; Gail Stetten; Chris Bird; Karin J. Blakemore

doi:10.1159/000098711

Use of T-cell antibodies for donor dosaging in a canine model of in utero hematopoietic stem cell transplantation

Scott M. Petersen, Mariya Gendelman, Kathleen M. Murphy, Michael Torbenson, Richard J. Jones, Janyne E. Althaus, Gail Stetten, Chris Bird, Karin J. Blakemore

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Aim: Microchimerism following canine in utero hematopoietic stem cell transplantation (IUHSCT) development of T-cell dosing regimens. Objective: To investigate the use of anti-T-cell antibodies for cell dosing of the donor graft in a canine model of IUHSCT. Study Design: Canine IUHSCT was performed by ultrasound-guided intraperitoneal injection in days 35-38 of fetal canines with CD34⁺ cells at doses of 4.5 × 10⁸ to 1.3 × 10⁹ cells/kg and T cells (CD3⁺ CD5⁺) at doses of 8 × 10⁶ to 8.8 × 10⁸ cells/kg. Postnatal studies included tissue histology and polymerase chain reaction-based chimerism analysis. Results: Term survival was 86-100%. Microchimerism (0-2%) was detected in five of eight recipients in multiple tissues. Histopathology revealed no evidence of graft-versus-host disease (GVHD). Conclusion: Canine IUHSCT is a useful model to investigate the role of donor T cells in engraftment and GVHD. IUHSCT at early gestational ages with high doses of donor T cells in the graft yields microchimerism in multiple tissues without GVHD.

Original language	English (US)
Pages (from-to)	175-179
Number of pages	5
Journal	Fetal Diagnosis and Therapy
Volume	22
Issue number	3
DOIs	https://doi.org/10.1159/000098711
State	Published - Apr 2007

Keywords

Bone marrow transplantation
Canine model
Hematopoietic stem cell

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Embryology
Radiology Nuclear Medicine and imaging
Obstetrics and Gynecology

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title = "Use of T-cell antibodies for donor dosaging in a canine model of in utero hematopoietic stem cell transplantation",

abstract = "Aim: Microchimerism following canine in utero hematopoietic stem cell transplantation (IUHSCT) development of T-cell dosing regimens. Objective: To investigate the use of anti-T-cell antibodies for cell dosing of the donor graft in a canine model of IUHSCT. Study Design: Canine IUHSCT was performed by ultrasound-guided intraperitoneal injection in days 35-38 of fetal canines with CD34+ cells at doses of 4.5 × 108 to 1.3 × 109 cells/kg and T cells (CD3+ CD5+) at doses of 8 × 106 to 8.8 × 108 cells/kg. Postnatal studies included tissue histology and polymerase chain reaction-based chimerism analysis. Results: Term survival was 86-100%. Microchimerism (0-2%) was detected in five of eight recipients in multiple tissues. Histopathology revealed no evidence of graft-versus-host disease (GVHD). Conclusion: Canine IUHSCT is a useful model to investigate the role of donor T cells in engraftment and GVHD. IUHSCT at early gestational ages with high doses of donor T cells in the graft yields microchimerism in multiple tissues without GVHD.",

keywords = "Bone marrow transplantation, Canine model, Hematopoietic stem cell",

author = "Petersen, {Scott M.} and Mariya Gendelman and Murphy, {Kathleen M.} and Michael Torbenson and Jones, {Richard J.} and Althaus, {Janyne E.} and Gail Stetten and Chris Bird and Blakemore, {Karin J.}",

year = "2007",

month = apr,

doi = "10.1159/000098711",

language = "English (US)",

volume = "22",

pages = "175--179",

journal = "Fetal Diagnosis and Therapy",

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T1 - Use of T-cell antibodies for donor dosaging in a canine model of in utero hematopoietic stem cell transplantation

AU - Petersen, Scott M.

AU - Gendelman, Mariya

AU - Murphy, Kathleen M.

AU - Torbenson, Michael

AU - Jones, Richard J.

AU - Althaus, Janyne E.

AU - Stetten, Gail

AU - Bird, Chris

AU - Blakemore, Karin J.

PY - 2007/4

Y1 - 2007/4

N2 - Aim: Microchimerism following canine in utero hematopoietic stem cell transplantation (IUHSCT) development of T-cell dosing regimens. Objective: To investigate the use of anti-T-cell antibodies for cell dosing of the donor graft in a canine model of IUHSCT. Study Design: Canine IUHSCT was performed by ultrasound-guided intraperitoneal injection in days 35-38 of fetal canines with CD34+ cells at doses of 4.5 × 108 to 1.3 × 109 cells/kg and T cells (CD3+ CD5+) at doses of 8 × 106 to 8.8 × 108 cells/kg. Postnatal studies included tissue histology and polymerase chain reaction-based chimerism analysis. Results: Term survival was 86-100%. Microchimerism (0-2%) was detected in five of eight recipients in multiple tissues. Histopathology revealed no evidence of graft-versus-host disease (GVHD). Conclusion: Canine IUHSCT is a useful model to investigate the role of donor T cells in engraftment and GVHD. IUHSCT at early gestational ages with high doses of donor T cells in the graft yields microchimerism in multiple tissues without GVHD.

AB - Aim: Microchimerism following canine in utero hematopoietic stem cell transplantation (IUHSCT) development of T-cell dosing regimens. Objective: To investigate the use of anti-T-cell antibodies for cell dosing of the donor graft in a canine model of IUHSCT. Study Design: Canine IUHSCT was performed by ultrasound-guided intraperitoneal injection in days 35-38 of fetal canines with CD34+ cells at doses of 4.5 × 108 to 1.3 × 109 cells/kg and T cells (CD3+ CD5+) at doses of 8 × 106 to 8.8 × 108 cells/kg. Postnatal studies included tissue histology and polymerase chain reaction-based chimerism analysis. Results: Term survival was 86-100%. Microchimerism (0-2%) was detected in five of eight recipients in multiple tissues. Histopathology revealed no evidence of graft-versus-host disease (GVHD). Conclusion: Canine IUHSCT is a useful model to investigate the role of donor T cells in engraftment and GVHD. IUHSCT at early gestational ages with high doses of donor T cells in the graft yields microchimerism in multiple tissues without GVHD.

KW - Bone marrow transplantation

KW - Canine model

KW - Hematopoietic stem cell

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Use of T-cell antibodies for donor dosaging in a canine model of in utero hematopoietic stem cell transplantation

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