Use of siRNA to study the function of MDC1 in DNA damage responses.

Zhenkun Lou, Junjie Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Small interfering RNA (siRNA) technology has emerged as a powerful genetic tool to investigate gene function in mammalian cells. Here we use siRNA to study a mediator of DNA damage-checkpoint protein 1 (MDC1), previously known as Kiaa0170 or NFBD1, in DNA damage responses. We show that MDC1 siRNA specifically and efficiently down-regulates MDC1 expression, resulting in defective radiation-induced apoptosis in A549 cells. Transfection with siRNA-resistant MDC1 restores radiation-induced apoptosis. These findings suggest that siRNA can be a very useful tool for the exploration of gene function in mammalian checkpoint responses.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalMethods in molecular biology (Clifton, N.J.)
Volume281
StatePublished - 2004

Fingerprint

Small Interfering RNA
DNA Damage
Proteins
Radiation
Apoptosis
Genes
Transfection
Down-Regulation
Technology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Use of siRNA to study the function of MDC1 in DNA damage responses. / Lou, Zhenkun; Chen, Junjie.

In: Methods in molecular biology (Clifton, N.J.), Vol. 281, 2004, p. 179-187.

Research output: Contribution to journalArticle

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