TY - JOUR
T1 - Use of polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis to detect a point mutation in the catalase-peroxidase gene (katG) of Mycobacterium tuberculosis
AU - Temesgen, Zelalem
AU - Satoh, Koji
AU - Uhl, James R.
AU - Kline, Bruce C.
AU - Cockerill, Franklin R.
PY - 1997/2
Y1 - 1997/2
N2 - We have previously reported that a significant percentage (44%) of isoniazid-resistant Mycobacterium tuberculosis strains carry an arginine to leucine mutation in codon 463 (R463L) in the catalase-peroxidase gene (katG). For the current study, we compared the utility of one mutation screening method, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis, with a reference method, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), to detect this mutation. The PCR-SSCP method detects mutations by electrophoretic mobility shifts of single-stranded DNA in nondenaturing polyacrylamide gels. The RFLP method detects a loss in an MspI restriction site which occurs when the R463L is present. Eighty one M. tuberculosis strains, including the wild type strain H37Rv, with isoniazid susceptibility in the range < 0.12 to > 32 μg ml-1 were evaluated. The results for the PCR-SSCP method were in complete agreement with the PCR-MspI RFLP reference method. Of 81 M. tuberculosis strains analysed, 13 showed mobility shifts by the PCR-SSCP method and all of those strains carried the R463L as detected by the PCR-MspI RFLP method. All of the remaining 54 strains had PCR-SSCP and PCR-MspI RFLP results identical to the wild type (R463) M. tuberculosis strain, H37Rv. It is concluded that the described PCR-SSCP is a reliable method for screening M. tuberculosis strains for the katG R463L mutation.
AB - We have previously reported that a significant percentage (44%) of isoniazid-resistant Mycobacterium tuberculosis strains carry an arginine to leucine mutation in codon 463 (R463L) in the catalase-peroxidase gene (katG). For the current study, we compared the utility of one mutation screening method, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis, with a reference method, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), to detect this mutation. The PCR-SSCP method detects mutations by electrophoretic mobility shifts of single-stranded DNA in nondenaturing polyacrylamide gels. The RFLP method detects a loss in an MspI restriction site which occurs when the R463L is present. Eighty one M. tuberculosis strains, including the wild type strain H37Rv, with isoniazid susceptibility in the range < 0.12 to > 32 μg ml-1 were evaluated. The results for the PCR-SSCP method were in complete agreement with the PCR-MspI RFLP reference method. Of 81 M. tuberculosis strains analysed, 13 showed mobility shifts by the PCR-SSCP method and all of those strains carried the R463L as detected by the PCR-MspI RFLP method. All of the remaining 54 strains had PCR-SSCP and PCR-MspI RFLP results identical to the wild type (R463) M. tuberculosis strain, H37Rv. It is concluded that the described PCR-SSCP is a reliable method for screening M. tuberculosis strains for the katG R463L mutation.
KW - Catalase-peroxidase gene
KW - Isoniazid
KW - Mycobacterium tuberculosis
KW - PCR-SSCP
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U2 - 10.1006/mcpr.1996.0077
DO - 10.1006/mcpr.1996.0077
M3 - Article
C2 - 9076716
AN - SCOPUS:0031079574
SN - 0890-8508
VL - 11
SP - 59
EP - 63
JO - Molecular and Cellular Probes
JF - Molecular and Cellular Probes
IS - 1
ER -