Use of fluorescence in situ hybridization to detect loss of chromosome 10 in astrocytomas

S. J. Dalrymple, J. F. Herath, S. R. Ritland, C. A. Moertel, R. B. Jenkins

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Models describing progression in the genetic derangement of glial tumors have shown loss of chromosome 10 to occur most frequently in high-grade lesions, suggesting that identification of this loss may be prognostically significant. Fluorescence in situ hybridization(FISH) analysis may be a valuable adjunct to histological grading if it can accurately detect this loss. In this paper the authors correlate results obtained from FISH, cytogenetic, molecular genetic, and flow cytometry analyses of a series of 39 brain specimens, including seven normal, two gliotic, and 30 neoplastic (one Grade II, one Grade III, and 28 Grade IV astrocytoma) specimens. Contiguous section of freshly resected surgical tissue were submitted for tissue culturing (karyotype) and touch preparation (FISH), snap-frozen (molecular genetic), or paraffin-embedded (histology and flow cytometry). Centromere- specific probes for chromosomes 10 and 12 were used for FISH analysis, and 19 restriction fragment length polymorphisms (two p-arm and 17 q-arm) and four microsatellite sequence polymorphisms (three p-arm and one q-arm) were used for molecular genetic analysis of chromosome 10. Findings showed FISH and loss of heterozygosity (LOH) analyses to be concordant in 33 of 38 specimens (sensitivity 94%, specificity 81%), with one specimen indeterminate on LOH analysis. Both FISH and LOH analyses were more sensitive at detecting chromosome 10 loss than conventional cytogenetic (karyotype) analysis. The authors conclude that FISH is a sensitive test for detecting chromosome 10 loss and ploidy in astrocytic tumors.

Original languageEnglish (US)
Pages (from-to)316-323
Number of pages8
JournalJournal of neurosurgery
Volume83
Issue number2
DOIs
StatePublished - Jan 1 1995

Keywords

  • astrocytoma
  • cytogenesis
  • flow cytometry
  • fluorescence in situ hybridization
  • molecular genetics

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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