Up-regulation of endothelial nitric oxide synthase through β2-adrenergic receptor - The role of a β-blocker with NO-releasing action

M. Jayachandran, T. Hayashi, D. Sumi, N. K. Thakur, H. Kano, L. J. Ignarro, A. Iguchi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We determined the existence and role of β2-adrenergic receptor in cultured BAECs through the effect of a β-blocker having NO releasing action; 3,4-dihydro-8(2-hydroxy-3-isopropylamino)-propoxy-3-nitroxy-2H-1-benzopyran; nipradilol on eNOS and eNOS regulatory protein caveolin-1. β2 receptor exists in BAECs. eNOS mRNA and protein were up-regulated by its treatment whereas those of caveolin were not altered considerably. This eNOS up-regulatory action was abolished by β2 receptor antagonist, ICI-118551. Increase of NO metabolites, protein and mRNA of eNOS was also partially inhibited by co-treatment of NOS inhibitor, L-NA with nipradilol. This is the first investigation of the action of non-selective β blocker on eNOS through β2 receptor. The drug increases NO on incubation with BAECs about 50% as a NO donor and about 50% as results of eNOS up-regulation.

Original languageEnglish (US)
Pages (from-to)589-594
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume280
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Nitric Oxide Synthase Type III
Adrenergic Receptors
Up-Regulation
Caveolins
Benzopyrans
Caveolin 1
Messenger RNA
Metabolites
Proteins
Pharmaceutical Preparations
nipradilol
ICI 118551

Keywords

  • β-adrenergic receptor antagonist
  • Caveolin-1
  • Endothelial nitric oxide synthase
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Up-regulation of endothelial nitric oxide synthase through β2-adrenergic receptor - The role of a β-blocker with NO-releasing action. / Jayachandran, M.; Hayashi, T.; Sumi, D.; Thakur, N. K.; Kano, H.; Ignarro, L. J.; Iguchi, A.

In: Biochemical and Biophysical Research Communications, Vol. 280, No. 3, 2001, p. 589-594.

Research output: Contribution to journalArticle

Jayachandran, M. ; Hayashi, T. ; Sumi, D. ; Thakur, N. K. ; Kano, H. ; Ignarro, L. J. ; Iguchi, A. / Up-regulation of endothelial nitric oxide synthase through β2-adrenergic receptor - The role of a β-blocker with NO-releasing action. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 280, No. 3. pp. 589-594.
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