Up-regulation of endothelial nitric oxide synthase through β2-adrenergic receptor - The role of a β-blocker with NO-releasing action

Muthuvel Jayachandran, Toshio Hayashi, Daigo Sumi, Navin Kumar Thakur, Hatsuyo Kano, Louis J. Ignarro, Akihisa Iguchi

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

We determined the existence and role of β2-adrenergic receptor in cultured BAECs through the effect of a β-blocker having NO releasing action; 3, 4-dihydro-8(2-hydroxy-3-isopropylamino)-propoxy-3-nitroxy-2H-1-benzopyran; nipradilol on eNOS and eNOS regulatory protein caveolin-1. β2receptor exists in BAECs. eNOS mRNA and protein were up-regulated by its treatment whereas those of caveolin were not altered considerably. This eNOS up-regulatory action was abolished by β2receptor antagonist, ICI-118551. Increase of NO metabolites, protein and mRNA of eNOS was also partially inhibited by co-treatment of NOS inhibitor, L-NA with nipradilol. This is the first investigation of the action of non-selective β blocker on eNOS through β2receptor. The drug increases NO on incubation with BAECs about 50% as a NO donor and about 50% as results of eNOS up-regulation.

Original languageEnglish (US)
Pages (from-to)589-594
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume280
Issue number3
DOIs
StatePublished - Jan 26 2001

Keywords

  • Caveo-lin-1
  • Endothelial nitric oxide synthase
  • Nitric oxide
  • β-adrenergic receptor antagonist

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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