@article{84ba2643589e4ca681f9eaa5c82af4a5,
title = "Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I",
abstract = "The multivesicular body (MVB) pathway is responsible for both the biosynthetic delivery of lysosomal hydrolases and the downregulation of numerous activated cell surface receptors which are degraded in the lysosome. We demonstrate that ubiquitination serves as a signal for sorting into the MVB pathway. In addition, we characterize a 350 kDa complex, ESCRT-I (composed of Vps23, Vps28, and Vps37), that recognizes ubiquitinated MVB cargo and whose function is required for sorting into MVB vesicles. This recognition event depends on a conserved UBC-like domain in Vps23. We propose that ESCRT-I represents a conserved component of the endosomal sorting machinery that functions in both yeast and mammalian cells to couple ubiquitin modification to protein sorting and receptor downregulation in the MVB pathway.",
author = "Katzmann, {David J.} and Markus Babst and Emr, {Scott D.}",
note = "Funding Information: We wish to thank Drs. Tom Stevens and Katherine Bowers for anti-DPAP B antiserum; Dr. Charles Zuker for anti-GFP antiserum; and Dr. Stefan Jentsch for the ubiquitin-GST plasmid. We also wish to thank Eden J. Estepa-Sabal for technical support and members of the Emr lab for helpful discussions, particularly Dr. Chris Stefan. We also wish to thank Dr. Randy Hampton and Nathan Bays, Dr. Linda Hicke and Dr. Mark Hochstrasser for helpful discussions and technical advice, and Drs. Chris Burd, Tamara Darsow, and Greg Odorizzi for critical reading of the manuscript. This work was supported by a grant from NIH to S.D.E. (CA 58689), a fellowship from the American Cancer Society (D.J.K.), and a fellowship from the Human Frontiers Science Program (M.B.). S.D.E. is supported as an Investigator of the Howard Hughes Medical Institute.",
year = "2001",
month = jul,
day = "27",
doi = "10.1016/S0092-8674(01)00434-2",
language = "English (US)",
volume = "106",
pages = "145--155",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",
}