U1 precursors: variant 3' flanking sequences are transcribed in human cells.

J. G. Patton, Eric D Wieben

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Using RNase protection and oligonucleotide hybridization experiments, we have shown that U1 precursors are derived by transcription of 3' flanking sequences. A labeled SP6 transcript of one of the true U1 genes (pD2) was able to protect a subset of the 3' flanking sequences present in HeLa cytoplasmic U1 RNA. However, not all U1 precursors were protected using this probe, suggesting that variant U1 precursor 3' tail sequences are expressed in HeLa cells. This conclusion has been confirmed by hybridization of HeLa RNA samples with specific oligonucleotide probes representing variant U1 3' flanking sequences. Interestingly, these variant tail sequences contain the putative Sm antigen binding site, A(U)3-6G. The conservation of this flanking sequence through evolution suggests a possible functional role for these precursor tails in ordering protein binding to U1 RNA.

Original languageEnglish (US)
Pages (from-to)175-182
Number of pages8
JournalJournal of Cell Biology
Volume104
Issue number2
StatePublished - Feb 1987

Fingerprint

3' Flanking Region
Tail
Oligonucleotide Probes
Ribonucleases
HeLa Cells
Protein Binding
Oligonucleotides
Binding Sites
RNA
Antigens
Genes
U1 small nuclear RNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

U1 precursors : variant 3' flanking sequences are transcribed in human cells. / Patton, J. G.; Wieben, Eric D.

In: Journal of Cell Biology, Vol. 104, No. 2, 02.1987, p. 175-182.

Research output: Contribution to journalArticle

@article{75b07fd8a6f345f19e3dbea748ea1267,
title = "U1 precursors: variant 3' flanking sequences are transcribed in human cells.",
abstract = "Using RNase protection and oligonucleotide hybridization experiments, we have shown that U1 precursors are derived by transcription of 3' flanking sequences. A labeled SP6 transcript of one of the true U1 genes (pD2) was able to protect a subset of the 3' flanking sequences present in HeLa cytoplasmic U1 RNA. However, not all U1 precursors were protected using this probe, suggesting that variant U1 precursor 3' tail sequences are expressed in HeLa cells. This conclusion has been confirmed by hybridization of HeLa RNA samples with specific oligonucleotide probes representing variant U1 3' flanking sequences. Interestingly, these variant tail sequences contain the putative Sm antigen binding site, A(U)3-6G. The conservation of this flanking sequence through evolution suggests a possible functional role for these precursor tails in ordering protein binding to U1 RNA.",
author = "Patton, {J. G.} and Wieben, {Eric D}",
year = "1987",
month = "2",
language = "English (US)",
volume = "104",
pages = "175--182",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "2",

}

TY - JOUR

T1 - U1 precursors

T2 - variant 3' flanking sequences are transcribed in human cells.

AU - Patton, J. G.

AU - Wieben, Eric D

PY - 1987/2

Y1 - 1987/2

N2 - Using RNase protection and oligonucleotide hybridization experiments, we have shown that U1 precursors are derived by transcription of 3' flanking sequences. A labeled SP6 transcript of one of the true U1 genes (pD2) was able to protect a subset of the 3' flanking sequences present in HeLa cytoplasmic U1 RNA. However, not all U1 precursors were protected using this probe, suggesting that variant U1 precursor 3' tail sequences are expressed in HeLa cells. This conclusion has been confirmed by hybridization of HeLa RNA samples with specific oligonucleotide probes representing variant U1 3' flanking sequences. Interestingly, these variant tail sequences contain the putative Sm antigen binding site, A(U)3-6G. The conservation of this flanking sequence through evolution suggests a possible functional role for these precursor tails in ordering protein binding to U1 RNA.

AB - Using RNase protection and oligonucleotide hybridization experiments, we have shown that U1 precursors are derived by transcription of 3' flanking sequences. A labeled SP6 transcript of one of the true U1 genes (pD2) was able to protect a subset of the 3' flanking sequences present in HeLa cytoplasmic U1 RNA. However, not all U1 precursors were protected using this probe, suggesting that variant U1 precursor 3' tail sequences are expressed in HeLa cells. This conclusion has been confirmed by hybridization of HeLa RNA samples with specific oligonucleotide probes representing variant U1 3' flanking sequences. Interestingly, these variant tail sequences contain the putative Sm antigen binding site, A(U)3-6G. The conservation of this flanking sequence through evolution suggests a possible functional role for these precursor tails in ordering protein binding to U1 RNA.

UR - http://www.scopus.com/inward/record.url?scp=0023294011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023294011&partnerID=8YFLogxK

M3 - Article

C2 - 3805120

AN - SCOPUS:0023294011

VL - 104

SP - 175

EP - 182

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 2

ER -