Tyrosinase Expression during Neuroblast Divisions Affects Later Pathfinding by Retinal Ganglion Cells

Carolyn A. Cronin, Amy B. Ryan, Edmund M. Talley, Heidi Scrable

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Occulocutaneous albinism is caused by mutations in the gene encoding the enzyme tyrosinase. Individuals with this disorder are predisposed to visual system deficits. We determined the critical period during development when tyrosinase expression is essential for the appropriate pathfinding of ganglion cell axons from the retina to the dorsal lateral geniculate nucleus. We used a line of mice with a Tyrosinase transgene, the expression of which is regulatable with the lac operator-repressor system, to restrict tyrosinase activity to discrete periods of embryogenesis. When tyrosinase was expressed throughout the period of neuroblast divisions that produce the ipsilaterally projecting ganglion cells, axonal projections innervated the same volume of the ipsilateral dorsal lateral geniculate nucleus of the thalamus as in normal mice. If tyrosinase expression ceased before the end of neuroblast divisions, or was not initiated until after they had begun, the degree of ipsilateral innervation was smaller, as in albino mice. Tyrosinase expression was not required during the entire period of pathfinding itself or during final maturation of the retinogeniculate pathway. Thus, tyrosinase appears to set up a signal early in visual system development that determines the pathway taken later by ganglion cell axons.

Original languageEnglish (US)
Pages (from-to)11692-11697
Number of pages6
JournalJournal of Neuroscience
Volume23
Issue number37
StatePublished - Dec 17 2003
Externally publishedYes

Fingerprint

Monophenol Monooxygenase
Retinal Ganglion Cells
Ganglia
Geniculate Bodies
Axons
Lac Repressors
Albinism
Lateral Thalamic Nuclei
Transgenes
Embryonic Development
Retina
Mutation
Enzymes

Keywords

  • Albino
  • Gene regulation
  • Lac repressor
  • Occulocutaneous albinism
  • Tyrosinase
  • Visual system development

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cronin, C. A., Ryan, A. B., Talley, E. M., & Scrable, H. (2003). Tyrosinase Expression during Neuroblast Divisions Affects Later Pathfinding by Retinal Ganglion Cells. Journal of Neuroscience, 23(37), 11692-11697.

Tyrosinase Expression during Neuroblast Divisions Affects Later Pathfinding by Retinal Ganglion Cells. / Cronin, Carolyn A.; Ryan, Amy B.; Talley, Edmund M.; Scrable, Heidi.

In: Journal of Neuroscience, Vol. 23, No. 37, 17.12.2003, p. 11692-11697.

Research output: Contribution to journalArticle

Cronin, CA, Ryan, AB, Talley, EM & Scrable, H 2003, 'Tyrosinase Expression during Neuroblast Divisions Affects Later Pathfinding by Retinal Ganglion Cells', Journal of Neuroscience, vol. 23, no. 37, pp. 11692-11697.
Cronin, Carolyn A. ; Ryan, Amy B. ; Talley, Edmund M. ; Scrable, Heidi. / Tyrosinase Expression during Neuroblast Divisions Affects Later Pathfinding by Retinal Ganglion Cells. In: Journal of Neuroscience. 2003 ; Vol. 23, No. 37. pp. 11692-11697.
@article{602f566876cd4958960abf58b7f23791,
title = "Tyrosinase Expression during Neuroblast Divisions Affects Later Pathfinding by Retinal Ganglion Cells",
abstract = "Occulocutaneous albinism is caused by mutations in the gene encoding the enzyme tyrosinase. Individuals with this disorder are predisposed to visual system deficits. We determined the critical period during development when tyrosinase expression is essential for the appropriate pathfinding of ganglion cell axons from the retina to the dorsal lateral geniculate nucleus. We used a line of mice with a Tyrosinase transgene, the expression of which is regulatable with the lac operator-repressor system, to restrict tyrosinase activity to discrete periods of embryogenesis. When tyrosinase was expressed throughout the period of neuroblast divisions that produce the ipsilaterally projecting ganglion cells, axonal projections innervated the same volume of the ipsilateral dorsal lateral geniculate nucleus of the thalamus as in normal mice. If tyrosinase expression ceased before the end of neuroblast divisions, or was not initiated until after they had begun, the degree of ipsilateral innervation was smaller, as in albino mice. Tyrosinase expression was not required during the entire period of pathfinding itself or during final maturation of the retinogeniculate pathway. Thus, tyrosinase appears to set up a signal early in visual system development that determines the pathway taken later by ganglion cell axons.",
keywords = "Albino, Gene regulation, Lac repressor, Occulocutaneous albinism, Tyrosinase, Visual system development",
author = "Cronin, {Carolyn A.} and Ryan, {Amy B.} and Talley, {Edmund M.} and Heidi Scrable",
year = "2003",
month = "12",
day = "17",
language = "English (US)",
volume = "23",
pages = "11692--11697",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "37",

}

TY - JOUR

T1 - Tyrosinase Expression during Neuroblast Divisions Affects Later Pathfinding by Retinal Ganglion Cells

AU - Cronin, Carolyn A.

AU - Ryan, Amy B.

AU - Talley, Edmund M.

AU - Scrable, Heidi

PY - 2003/12/17

Y1 - 2003/12/17

N2 - Occulocutaneous albinism is caused by mutations in the gene encoding the enzyme tyrosinase. Individuals with this disorder are predisposed to visual system deficits. We determined the critical period during development when tyrosinase expression is essential for the appropriate pathfinding of ganglion cell axons from the retina to the dorsal lateral geniculate nucleus. We used a line of mice with a Tyrosinase transgene, the expression of which is regulatable with the lac operator-repressor system, to restrict tyrosinase activity to discrete periods of embryogenesis. When tyrosinase was expressed throughout the period of neuroblast divisions that produce the ipsilaterally projecting ganglion cells, axonal projections innervated the same volume of the ipsilateral dorsal lateral geniculate nucleus of the thalamus as in normal mice. If tyrosinase expression ceased before the end of neuroblast divisions, or was not initiated until after they had begun, the degree of ipsilateral innervation was smaller, as in albino mice. Tyrosinase expression was not required during the entire period of pathfinding itself or during final maturation of the retinogeniculate pathway. Thus, tyrosinase appears to set up a signal early in visual system development that determines the pathway taken later by ganglion cell axons.

AB - Occulocutaneous albinism is caused by mutations in the gene encoding the enzyme tyrosinase. Individuals with this disorder are predisposed to visual system deficits. We determined the critical period during development when tyrosinase expression is essential for the appropriate pathfinding of ganglion cell axons from the retina to the dorsal lateral geniculate nucleus. We used a line of mice with a Tyrosinase transgene, the expression of which is regulatable with the lac operator-repressor system, to restrict tyrosinase activity to discrete periods of embryogenesis. When tyrosinase was expressed throughout the period of neuroblast divisions that produce the ipsilaterally projecting ganglion cells, axonal projections innervated the same volume of the ipsilateral dorsal lateral geniculate nucleus of the thalamus as in normal mice. If tyrosinase expression ceased before the end of neuroblast divisions, or was not initiated until after they had begun, the degree of ipsilateral innervation was smaller, as in albino mice. Tyrosinase expression was not required during the entire period of pathfinding itself or during final maturation of the retinogeniculate pathway. Thus, tyrosinase appears to set up a signal early in visual system development that determines the pathway taken later by ganglion cell axons.

KW - Albino

KW - Gene regulation

KW - Lac repressor

KW - Occulocutaneous albinism

KW - Tyrosinase

KW - Visual system development

UR - http://www.scopus.com/inward/record.url?scp=0345802825&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345802825&partnerID=8YFLogxK

M3 - Article

C2 - 14684871

AN - SCOPUS:0345802825

VL - 23

SP - 11692

EP - 11697

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 37

ER -