Twisted is a conserved extracellular BMP antagonist

Jeffrey J. Ross, Osamu Shimmi, Peter Vilmos, Anna Petryk, Hyon Kim, Karin Gaudenz, Spencer Hermanson, Stephen C Ekker, Michael B. O'connor, J. Lawrence Marsh

Research output: Contribution to journalArticle

227 Citations (Scopus)

Abstract

Bone morphogenetic protein (BMP) signalling regulates embryonic dorsal-ventral cell fate decisions in flies, frogs and fish 1. BMP activity is controlled by several secreted factors including the antagonists chordin and short gastrulation (SOG) 2,3. Here we show that a second secreted protein, Twisted gastrulation (Tsg) 4, enhances the antagonistic activity of Sog/chordin. In Drosophila, visualization of BMP signalling using anti-phospho-Smad staining 5 shows that the tsg and sog loss-of-function phenotypes are very similar. In S2 cells and imaginal discs, TSG and SOG together make a more effective inhibitor of BMP signalling than either of them alone. Blocking Tsg function in zebrafish with morpholino oligonucleotides causes ventralization similar to that produced by chordin mutants. Co-injection of sub-inhibitory levels of morpholines directed against both Tsg and chordin synergistically enhances the penetrance of the ventralized phenotype. We show that Tsgs from different species are functionally equivalent, and conclude that Tsg is a conserved protein that functions with SOG/chordin to antagonize BMP signalling.

Original languageEnglish (US)
Pages (from-to)479-483
Number of pages5
JournalNature
Volume410
Issue number6827
DOIs
StatePublished - Mar 22 2001
Externally publishedYes

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Gastrulation
Bone Morphogenetic Proteins
Morpholines
Imaginal Discs
Phenotype
Morpholinos
Penetrance
Zebrafish
Diptera
Anura
Drosophila
Fishes
Proteins
chordin
Staining and Labeling
Injections

ASJC Scopus subject areas

  • General

Cite this

Ross, J. J., Shimmi, O., Vilmos, P., Petryk, A., Kim, H., Gaudenz, K., ... Marsh, J. L. (2001). Twisted is a conserved extracellular BMP antagonist. Nature, 410(6827), 479-483. https://doi.org/10.1038/35068578

Twisted is a conserved extracellular BMP antagonist. / Ross, Jeffrey J.; Shimmi, Osamu; Vilmos, Peter; Petryk, Anna; Kim, Hyon; Gaudenz, Karin; Hermanson, Spencer; Ekker, Stephen C; O'connor, Michael B.; Marsh, J. Lawrence.

In: Nature, Vol. 410, No. 6827, 22.03.2001, p. 479-483.

Research output: Contribution to journalArticle

Ross, JJ, Shimmi, O, Vilmos, P, Petryk, A, Kim, H, Gaudenz, K, Hermanson, S, Ekker, SC, O'connor, MB & Marsh, JL 2001, 'Twisted is a conserved extracellular BMP antagonist', Nature, vol. 410, no. 6827, pp. 479-483. https://doi.org/10.1038/35068578
Ross JJ, Shimmi O, Vilmos P, Petryk A, Kim H, Gaudenz K et al. Twisted is a conserved extracellular BMP antagonist. Nature. 2001 Mar 22;410(6827):479-483. https://doi.org/10.1038/35068578
Ross, Jeffrey J. ; Shimmi, Osamu ; Vilmos, Peter ; Petryk, Anna ; Kim, Hyon ; Gaudenz, Karin ; Hermanson, Spencer ; Ekker, Stephen C ; O'connor, Michael B. ; Marsh, J. Lawrence. / Twisted is a conserved extracellular BMP antagonist. In: Nature. 2001 ; Vol. 410, No. 6827. pp. 479-483.
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