Tryptic peptide comparison of Ia antigen α and β polypeptides from the I-A Mutant B6.C-H-2bm12 and its congenic parental strain B6

David J. McKean, Roger W. Melvold, Chella David

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Abstract

Previous studies of the B6.C-H-2 bm12 (bm12) strain have demonstrated the presence of a mutation localized to the I-A subregion of the mouse H-2 major histocompatibility complex. This mutation has been shown to be responsible for defects in Ir-gene function and in Ia and MLR determinants. A comparison of the molecular size of the bm12 mutant and the parental B6 Ia-antigen component polypeptides failed to demonstrate any differences in the α and β polypeptides. Thus, no major structural additions or deletions are present in the Ia α and β chain polypeptide or carbohydrate structure. A significant decrease in the amount of invariant (31K) polypeptide was, however, consistently observed in the bm12 Ia antigen preparations. Tryptic peptide comparisons of 14C B6 and 3H bm 12 α and β polypeptides demonstrated a limited number of peptide differences in the bm12 β polypeptide but none in the bm12 α polypeptide. The significance of these biochemical mutations and altered biological phenomena are discussed in relation to a model of the immunological interaction sites on Ia antigens.

Original languageEnglish (US)
Pages (from-to)41-51
Number of pages11
JournalImmunogenetics
Volume14
Issue number1-2
DOIs
StatePublished - Sep 1981

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Histocompatibility Antigens Class II
Peptides
Mutation
Immunological Models
Biological Phenomena
Major Histocompatibility Complex
Carbohydrates

ASJC Scopus subject areas

  • Immunology
  • Genetics

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Tryptic peptide comparison of Ia antigen α and β polypeptides from the I-A Mutant B6.C-H-2bm12 and its congenic parental strain B6. / McKean, David J.; Melvold, Roger W.; David, Chella.

In: Immunogenetics, Vol. 14, No. 1-2, 09.1981, p. 41-51.

Research output: Contribution to journalArticle

McKean, David J. ; Melvold, Roger W. ; David, Chella. / Tryptic peptide comparison of Ia antigen α and β polypeptides from the I-A Mutant B6.C-H-2bm12 and its congenic parental strain B6. In: Immunogenetics. 1981 ; Vol. 14, No. 1-2. pp. 41-51.
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