Triglyceride synthesis in rat skeletal muscles

Z. K. Guo, B. Buergeta, Michael Dennis Jensen

Research output: Contribution to journalArticle

Abstract

In order to gain first insight into triglyceride (TG) synthesis in skeletal muscle, conscious Sprague Dawley rats were intravenously infused with [U-13C]palmitate and [1-13C]oleate simultaneously at 12 and 18 nmol·kg· min respectively, or at quadrupled rates. Gastrocnemius and soleus muscles were biopsied after 2 and 4 h of the infusion. Muscle TG and non-esterified fatty acid (NEFA) were purified by HPLC and adsorption chromatography. 13C enrichments and concentrations of the constituent palmitate and oleate were determined by gas chromatography/combustion/isotope ratio mass spectrometry. Muscle TG fractional synthesis rates (FSR) were calculated using NEFA as the precursor pool: Gastrocnemius 0.267±0.075·h by [U-13C]palmitate and 0.278±0.049·h by [1-13C]oleate; Soleus 0.100±0.030·h by [U-13C]palmitate and 0.075±0.013·h by [1-13C]oleate. TG fatty acid pool sizes of gastrocnemius and soleus were: palmitate 4.4±0.8 and 18.0±3.1 μmol·g dry muscle respectively (P=0.002); Oleate 3.8±0.8 and 21.8±4.9 μmol·g dry muscle respectively (P=0.006). The data suggested that plasma free fatty acids are an important source for muscle TG synthesis and muscle TG is extremely active metabolically; TG FSRs in the two muscles are different (P=0.039 by [U-13C]palmitate and P=0.022 by [1-13C]oleate) and the differences are due to the much larger TG pool in soleus than that in gastrocnemius muscle.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

Fingerprint

Muscle
skeletal muscle
Rats
Palmitates
Skeletal Muscle
Triglycerides
Oleic Acid
palmitates
triacylglycerols
muscles
synthesis
oleic acid
rats
Muscles
Fatty Acids
free fatty acids
fractional synthesis rate
Nonesterified Fatty Acids
Isotopes
Gas Chromatography

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Triglyceride synthesis in rat skeletal muscles. / Guo, Z. K.; Buergeta, B.; Jensen, Michael Dennis.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Guo, ZK, Buergeta, B & Jensen, MD 1997, 'Triglyceride synthesis in rat skeletal muscles', FASEB Journal, vol. 11, no. 3.
Guo, Z. K. ; Buergeta, B. ; Jensen, Michael Dennis. / Triglyceride synthesis in rat skeletal muscles. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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