Transport proteins NHA1 and NHA2 are essential for survival, but have distinct transport modalities

Venkateswara R. Chintapalli, Akira Kato, Louise Henderson, Taku Hirata, Debra J. Woods, Gayle Overend, Shireen A. Davies, Michael F Romero, Julian A T Dow

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The cation/proton antiporter (CPA) family includes the well-known sodium/proton exchanger (NHE; SLC9A) family of Na+/H+ exchangers, and the more recently discovered and less well understood CPA2s (SLC9B), found widely in living organisms. In Drosophila, as in humans, they are represented by two genes, Nha1 (Slc9b1) and Nha2 (Slc9b2), which are enriched and functionally significant in renal tubules. The importance of their role in organismal survival has not been investigated in animals, however. Here we show that single RNAi knockdowns of either Nha1 or Nha2 reduce survival and in combination are lethal. Knockdown of either gene alone results in up-regulation of the other, suggesting functional complementation of the two genes. Under salt stress, knockdown of either gene decreases survival, demonstrating a key role for the CPA2 family in ion homeostasis. This is specific to Na+ stress; survival on K+ intoxication is not affected by sodium/hydrogen antiporter (NHA) knockdown. A direct functional assay in Xenopus oocytes shows that Nha2 acts as a Na+/H+ exchanger. In contrast, Nha1 expressed in Xenopus oocytes shows strong Cl. conductance and acts as a H+-Cl- cotransporter. The activity of Nha1 is inhibited by chloride-binding competitors 4,4′-diiso-thiocyano-2,2′-disulfonic acid stilbene and 4,4′-dibenzamido- 2,2′-stilbenedisulphonate. Salt stress induces a massive up-regulation of NHA gene expression not in themajor osmoregulatory tissues of the alimentary canal, but in the crop, cuticle, and associated tissues. Thus, it is necessary to revise the classical viewof the coordination of different tissues in the coordination of the response to osmoregulatory stress.

Original languageEnglish (US)
Pages (from-to)11720-11725
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number37
DOIs
StatePublished - Sep 15 2015

Fingerprint

Sodium-Hydrogen Antiporter
Carrier Proteins
Gene Knockdown Techniques
Survival
Xenopus
Oocytes
Protons
Up-Regulation
Salts
Antiporters
Stilbenes
Gene Expression Regulation
RNA Interference
Genes
Drosophila
Cations
Chlorides
Homeostasis
Sodium
Ions

Keywords

  • Drosophila
  • Exchanger
  • Genetics
  • Physiology
  • Transport

ASJC Scopus subject areas

  • General

Cite this

Transport proteins NHA1 and NHA2 are essential for survival, but have distinct transport modalities. / Chintapalli, Venkateswara R.; Kato, Akira; Henderson, Louise; Hirata, Taku; Woods, Debra J.; Overend, Gayle; Davies, Shireen A.; Romero, Michael F; Dow, Julian A T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 37, 15.09.2015, p. 11720-11725.

Research output: Contribution to journalArticle

Chintapalli, Venkateswara R. ; Kato, Akira ; Henderson, Louise ; Hirata, Taku ; Woods, Debra J. ; Overend, Gayle ; Davies, Shireen A. ; Romero, Michael F ; Dow, Julian A T. / Transport proteins NHA1 and NHA2 are essential for survival, but have distinct transport modalities. In: Proceedings of the National Academy of Sciences of the United States of America. 2015 ; Vol. 112, No. 37. pp. 11720-11725.
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AU - Hirata, Taku

AU - Woods, Debra J.

AU - Overend, Gayle

AU - Davies, Shireen A.

AU - Romero, Michael F

AU - Dow, Julian A T

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