Transport and metabolism of pantothenic acid by rat kidney

Larry M Karnitz, Carol J. Gross, Lavell M. Henderson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Transport of [14C]pantothenic acid was studied using brush-border membrane vesicles prepared from rat kidney. In the presence of a Na+ gradient an accumulation of pantothenic acid 3-fold above equilibrium was observed. The Km and Vmax found were 7.30 μM and 23.8 pmol/mg protein per min, respectively. Isolated perfused rat kidneys were employed to study excretion of pantothenic acid at various concentrations in the perfusate. At physiological plasma concentrations, the filtered pantothenic acid was largely reabsorbed by the active process observed in the vesicles. At higher concentrations, pantothenic acid was found to undergo tubular secretion. Penicillin inhibited this secretory process indicating that both compounds share a secretory mechanism. Live animal studies indicated that the only compound excreted after injection of [14C]pantothenic acid was free pantothenic acid. After 1 week only 38% of the administered dose was excreted in the urine, indicating that effective conservation was taking place in the whole animal.

Original languageEnglish (US)
Pages (from-to)486-492
Number of pages7
JournalBBA - Biomembranes
Volume769
Issue number2
DOIs
StatePublished - Jan 25 1984
Externally publishedYes

Fingerprint

Pantothenic Acid
Metabolism
Rats
Kidney
Animals
Secretory Pathway
Brushes
Microvilli
Penicillins
Conservation
Urine
Membranes
Plasmas
Injections

Keywords

  • (Rat kidney)
  • Pantothenic acid
  • Vitamin transport

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Transport and metabolism of pantothenic acid by rat kidney. / Karnitz, Larry M; Gross, Carol J.; Henderson, Lavell M.

In: BBA - Biomembranes, Vol. 769, No. 2, 25.01.1984, p. 486-492.

Research output: Contribution to journalArticle

Karnitz, Larry M ; Gross, Carol J. ; Henderson, Lavell M. / Transport and metabolism of pantothenic acid by rat kidney. In: BBA - Biomembranes. 1984 ; Vol. 769, No. 2. pp. 486-492.
@article{b4c8445a92b644d0acaf84bbc69ba01e,
title = "Transport and metabolism of pantothenic acid by rat kidney",
abstract = "Transport of [14C]pantothenic acid was studied using brush-border membrane vesicles prepared from rat kidney. In the presence of a Na+ gradient an accumulation of pantothenic acid 3-fold above equilibrium was observed. The Km and Vmax found were 7.30 μM and 23.8 pmol/mg protein per min, respectively. Isolated perfused rat kidneys were employed to study excretion of pantothenic acid at various concentrations in the perfusate. At physiological plasma concentrations, the filtered pantothenic acid was largely reabsorbed by the active process observed in the vesicles. At higher concentrations, pantothenic acid was found to undergo tubular secretion. Penicillin inhibited this secretory process indicating that both compounds share a secretory mechanism. Live animal studies indicated that the only compound excreted after injection of [14C]pantothenic acid was free pantothenic acid. After 1 week only 38{\%} of the administered dose was excreted in the urine, indicating that effective conservation was taking place in the whole animal.",
keywords = "(Rat kidney), Pantothenic acid, Vitamin transport",
author = "Karnitz, {Larry M} and Gross, {Carol J.} and Henderson, {Lavell M.}",
year = "1984",
month = "1",
day = "25",
doi = "10.1016/0005-2736(84)90334-1",
language = "English (US)",
volume = "769",
pages = "486--492",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Transport and metabolism of pantothenic acid by rat kidney

AU - Karnitz, Larry M

AU - Gross, Carol J.

AU - Henderson, Lavell M.

PY - 1984/1/25

Y1 - 1984/1/25

N2 - Transport of [14C]pantothenic acid was studied using brush-border membrane vesicles prepared from rat kidney. In the presence of a Na+ gradient an accumulation of pantothenic acid 3-fold above equilibrium was observed. The Km and Vmax found were 7.30 μM and 23.8 pmol/mg protein per min, respectively. Isolated perfused rat kidneys were employed to study excretion of pantothenic acid at various concentrations in the perfusate. At physiological plasma concentrations, the filtered pantothenic acid was largely reabsorbed by the active process observed in the vesicles. At higher concentrations, pantothenic acid was found to undergo tubular secretion. Penicillin inhibited this secretory process indicating that both compounds share a secretory mechanism. Live animal studies indicated that the only compound excreted after injection of [14C]pantothenic acid was free pantothenic acid. After 1 week only 38% of the administered dose was excreted in the urine, indicating that effective conservation was taking place in the whole animal.

AB - Transport of [14C]pantothenic acid was studied using brush-border membrane vesicles prepared from rat kidney. In the presence of a Na+ gradient an accumulation of pantothenic acid 3-fold above equilibrium was observed. The Km and Vmax found were 7.30 μM and 23.8 pmol/mg protein per min, respectively. Isolated perfused rat kidneys were employed to study excretion of pantothenic acid at various concentrations in the perfusate. At physiological plasma concentrations, the filtered pantothenic acid was largely reabsorbed by the active process observed in the vesicles. At higher concentrations, pantothenic acid was found to undergo tubular secretion. Penicillin inhibited this secretory process indicating that both compounds share a secretory mechanism. Live animal studies indicated that the only compound excreted after injection of [14C]pantothenic acid was free pantothenic acid. After 1 week only 38% of the administered dose was excreted in the urine, indicating that effective conservation was taking place in the whole animal.

KW - (Rat kidney)

KW - Pantothenic acid

KW - Vitamin transport

UR - http://www.scopus.com/inward/record.url?scp=0021363447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021363447&partnerID=8YFLogxK

U2 - 10.1016/0005-2736(84)90334-1

DO - 10.1016/0005-2736(84)90334-1

M3 - Article

VL - 769

SP - 486

EP - 492

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

SN - 0005-2736

IS - 2

ER -