Transport and metabolism of pantothenic acid by rat kidney

Larry M. Karnitz; Carol J. Gross; Lavell M. Henderson

doi:10.1016/0005-2736(84)90334-1

Transport and metabolism of pantothenic acid by rat kidney

Larry M. Karnitz, Carol J. Gross, Lavell M. Henderson

Oncology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Transport of [¹⁴C]pantothenic acid was studied using brush-border membrane vesicles prepared from rat kidney. In the presence of a Na⁺ gradient an accumulation of pantothenic acid 3-fold above equilibrium was observed. The K_m and V_max found were 7.30 μM and 23.8 pmol/mg protein per min, respectively. Isolated perfused rat kidneys were employed to study excretion of pantothenic acid at various concentrations in the perfusate. At physiological plasma concentrations, the filtered pantothenic acid was largely reabsorbed by the active process observed in the vesicles. At higher concentrations, pantothenic acid was found to undergo tubular secretion. Penicillin inhibited this secretory process indicating that both compounds share a secretory mechanism. Live animal studies indicated that the only compound excreted after injection of [¹⁴C]pantothenic acid was free pantothenic acid. After 1 week only 38% of the administered dose was excreted in the urine, indicating that effective conservation was taking place in the whole animal.

Original language	English (US)
Pages (from-to)	486-492
Number of pages	7
Journal	BBA - Biomembranes
Volume	769
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(84)90334-1
State	Published - Jan 25 1984

Keywords

(Rat kidney)
Pantothenic acid
Vitamin transport

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(84)90334-1

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title = "Transport and metabolism of pantothenic acid by rat kidney",

abstract = "Transport of [14C]pantothenic acid was studied using brush-border membrane vesicles prepared from rat kidney. In the presence of a Na+ gradient an accumulation of pantothenic acid 3-fold above equilibrium was observed. The Km and Vmax found were 7.30 μM and 23.8 pmol/mg protein per min, respectively. Isolated perfused rat kidneys were employed to study excretion of pantothenic acid at various concentrations in the perfusate. At physiological plasma concentrations, the filtered pantothenic acid was largely reabsorbed by the active process observed in the vesicles. At higher concentrations, pantothenic acid was found to undergo tubular secretion. Penicillin inhibited this secretory process indicating that both compounds share a secretory mechanism. Live animal studies indicated that the only compound excreted after injection of [14C]pantothenic acid was free pantothenic acid. After 1 week only 38% of the administered dose was excreted in the urine, indicating that effective conservation was taking place in the whole animal.",

keywords = "(Rat kidney), Pantothenic acid, Vitamin transport",

author = "Karnitz, {Larry M.} and Gross, {Carol J.} and Henderson, {Lavell M.}",

note = "Funding Information: This study was supported by USPHS, NIH Grant 5 R01-AM19012.",

year = "1984",

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doi = "10.1016/0005-2736(84)90334-1",

language = "English (US)",

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T1 - Transport and metabolism of pantothenic acid by rat kidney

AU - Karnitz, Larry M.

AU - Gross, Carol J.

AU - Henderson, Lavell M.

N1 - Funding Information: This study was supported by USPHS, NIH Grant 5 R01-AM19012.

PY - 1984/1/25

Y1 - 1984/1/25

N2 - Transport of [14C]pantothenic acid was studied using brush-border membrane vesicles prepared from rat kidney. In the presence of a Na+ gradient an accumulation of pantothenic acid 3-fold above equilibrium was observed. The Km and Vmax found were 7.30 μM and 23.8 pmol/mg protein per min, respectively. Isolated perfused rat kidneys were employed to study excretion of pantothenic acid at various concentrations in the perfusate. At physiological plasma concentrations, the filtered pantothenic acid was largely reabsorbed by the active process observed in the vesicles. At higher concentrations, pantothenic acid was found to undergo tubular secretion. Penicillin inhibited this secretory process indicating that both compounds share a secretory mechanism. Live animal studies indicated that the only compound excreted after injection of [14C]pantothenic acid was free pantothenic acid. After 1 week only 38% of the administered dose was excreted in the urine, indicating that effective conservation was taking place in the whole animal.

AB - Transport of [14C]pantothenic acid was studied using brush-border membrane vesicles prepared from rat kidney. In the presence of a Na+ gradient an accumulation of pantothenic acid 3-fold above equilibrium was observed. The Km and Vmax found were 7.30 μM and 23.8 pmol/mg protein per min, respectively. Isolated perfused rat kidneys were employed to study excretion of pantothenic acid at various concentrations in the perfusate. At physiological plasma concentrations, the filtered pantothenic acid was largely reabsorbed by the active process observed in the vesicles. At higher concentrations, pantothenic acid was found to undergo tubular secretion. Penicillin inhibited this secretory process indicating that both compounds share a secretory mechanism. Live animal studies indicated that the only compound excreted after injection of [14C]pantothenic acid was free pantothenic acid. After 1 week only 38% of the administered dose was excreted in the urine, indicating that effective conservation was taking place in the whole animal.

KW - (Rat kidney)

KW - Pantothenic acid

KW - Vitamin transport

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IS - 2

ER -

Transport and metabolism of pantothenic acid by rat kidney

Abstract

Keywords

ASJC Scopus subject areas

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