Transplanted clonal neural stem-like cells respond to remote photic stimulation following incorporation within the suprachiasmatic nucleus

Piotr Zlomanczuk, Maciej Mrugala, Horacio O. De La Iglesia, Vaclav Ourednik, Peter J. Quesenberry, Evan Y. Snyder, William J. Schwartz

Research output: Contribution to journalArticle

17 Scopus citations


Multipotent neural stem-like cells (NSCs) obtained from one brain region and transplanted to another region appear to differentiate into neuronal and glial phenotypes indigenous to the implantation site. Whether these donor-derived cells are appropriately integrated remains unanswered. In order to test this possibility, we exploited the suprachiasmatic nucleus (SCN) of the hypothalamus, site of a known circadian clock, as a novel engraftment target. When a clone of NSCs initially derived from neonatal mouse cerebellum was transplanted into mouse embryos, the cells incorporated within the SCN over a narrow gestational window that corresponded to the conclusion of SCN neurogenesis. Immunocytochemical staining suggested that donor-derived cells in the SCN synthesized a peptide neurotransmitter (arginine vasopressin) characteristic of SCN neurons. Donor-derived SCN cells reacted to light pulses by expressing immunoreactive c-Fos protein in a pattern that is appropriate for native SCN cells. This region-specific and physiologically appropriate response to the natural stimulation of a remote sensory input implies that donor-derived and endogenous cells formed true SCN chimeras, suggesting that exogenous NSCs engrafted to ectopic locations can integrate in a meaningful fashion.

Original languageEnglish (US)
Pages (from-to)162-168
Number of pages7
JournalExperimental Neurology
Issue number2
StatePublished - Jan 1 2002



  • Circadian rhythms
  • Neural stern cells
  • Neurogenesis
  • Suprachiasmatic nucleus
  • Transplantation
  • c-Fos

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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