Transforming growth factor β1 inhibition of p34cdc2 phosphorylation and histone H1 kinase activity is associated with G1/S-phase growth arrest

Philip H. Howe, Giulio Draetta, Edward B Leof

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Transforming growth factor β1 (TGFβ1) is a potent inhibitor of epithelial cell proliferation. We present data which indicate that epithelial cell proliferation is inhibited when TGFβ1 is added throughout the prereplicative G1 phase. Cultures become reversibly blocked in late G1 at the G1/S-phase boundary. The inhibitory effects of TGFβ1 on cell growth occur in the presence of the RNA synthesis inhibitor 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole. Associated with this inhibitory effect is a decrease in the phosphorylation and histone H1 kinase activity of the p34cdc2 protein kinase. These data suggest that TGFβ1 growth inhibition in epithelial cells involves the regulation of p34cdc2 activity at the G1/S transition.

Original languageEnglish (US)
Pages (from-to)1185-1194
Number of pages10
JournalMolecular and Cellular Biology
Volume11
Issue number3
StatePublished - Mar 1991
Externally publishedYes

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G1 Phase
Transforming Growth Factors
S Phase
Phosphorylation
Epithelial Cells
Growth
Cell Proliferation
CDC2 Protein Kinase
Nucleic Acid Synthesis Inhibitors
Protein Kinases
histone H1 kinase

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Transforming growth factor β1 inhibition of p34cdc2 phosphorylation and histone H1 kinase activity is associated with G1/S-phase growth arrest. / Howe, Philip H.; Draetta, Giulio; Leof, Edward B.

In: Molecular and Cellular Biology, Vol. 11, No. 3, 03.1991, p. 1185-1194.

Research output: Contribution to journalArticle

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