Abstract
Transforming growth factor β1 (TGFβ1) is a potent inhibitor of epithelial cell proliferation. We present data which indicate that epithelial cell proliferation is inhibited when TGFβ1 is added throughout the prereplicative G1 phase. Cultures become reversibly blocked in late G1 at the G1/S-phase boundary. The inhibitory effects of TGFβ1 on cell growth occur in the presence of the RNA synthesis inhibitor 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole. Associated with this inhibitory effect is a decrease in the phosphorylation and histone H1 kinase activity of the p34cdc2 protein kinase. These data suggest that TGFβ1 growth inhibition in epithelial cells involves the regulation of p34cdc2 activity at the G1/S transition.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1185-1194 |
| Number of pages | 10 |
| Journal | Molecular and Cellular Biology |
| Volume | 11 |
| Issue number | 3 |
| State | Published - Mar 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Genetics
- Molecular Biology
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Transforming growth factor β1 inhibition of p34cdc2 phosphorylation and histone H1 kinase activity is associated with G1/S-phase growth arrest. / Howe, Philip H.; Draetta, Giulio; Leof, Edward B.
In: Molecular and Cellular Biology, Vol. 11, No. 3, 03.1991, p. 1185-1194.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transforming growth factor β1 inhibition of p34cdc2 phosphorylation and histone H1 kinase activity is associated with G1/S-phase growth arrest
AU - Howe, Philip H.
AU - Draetta, Giulio
AU - Leof, Edward B
PY - 1991/3
Y1 - 1991/3
N2 - Transforming growth factor β1 (TGFβ1) is a potent inhibitor of epithelial cell proliferation. We present data which indicate that epithelial cell proliferation is inhibited when TGFβ1 is added throughout the prereplicative G1 phase. Cultures become reversibly blocked in late G1 at the G1/S-phase boundary. The inhibitory effects of TGFβ1 on cell growth occur in the presence of the RNA synthesis inhibitor 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole. Associated with this inhibitory effect is a decrease in the phosphorylation and histone H1 kinase activity of the p34cdc2 protein kinase. These data suggest that TGFβ1 growth inhibition in epithelial cells involves the regulation of p34cdc2 activity at the G1/S transition.
AB - Transforming growth factor β1 (TGFβ1) is a potent inhibitor of epithelial cell proliferation. We present data which indicate that epithelial cell proliferation is inhibited when TGFβ1 is added throughout the prereplicative G1 phase. Cultures become reversibly blocked in late G1 at the G1/S-phase boundary. The inhibitory effects of TGFβ1 on cell growth occur in the presence of the RNA synthesis inhibitor 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole. Associated with this inhibitory effect is a decrease in the phosphorylation and histone H1 kinase activity of the p34cdc2 protein kinase. These data suggest that TGFβ1 growth inhibition in epithelial cells involves the regulation of p34cdc2 activity at the G1/S transition.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025967749&partnerID=8YFLogxK
M3 - Article
C2 - 1996085
AN - SCOPUS:0025967749
VL - 11
SP - 1185
EP - 1194
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 3
ER -