Transcriptional specialization of human dendritic cell subsets in response to microbial vaccines

Romain Banchereau, Nicole Baldwin, Alma Martina Cepika, Shruti Athale, Yaming Xue, Chun I. Yu, Patrick Metang, Abhilasha Cheruku, Isabelle Berthier, Ingrid Gayet, Yuanyuan Wang, Marina Ohouo, Lu Ann Snipes, Hui Xu, Gerlinde Obermoser, Derek Blankenship, Sangkon Oh, Octavio Ramilo, Damien Chaussabel, Jacques BanchereauKarolina Palucka, Virginia Pascual

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The mechanisms by which microbial vaccines interact with human APCs remain elusive. Herein, we describe the transcriptional programs induced in human DCs by pathogens, innate receptor ligands and vaccines. Exposure of DCs to influenza, Salmonella enterica and Staphylococcus aureus allows us to build a modular framework containing 204 transcript clusters. We use this framework to characterize the responses of human monocytes, monocyte-derived DCs and blood DC subsets to 13 vaccines. Different vaccines induce distinct transcriptional programs based on pathogen type, adjuvant formulation and APC targeted. Fluzone, Pneumovax and Gardasil, respectively, activate monocyte-derived DCs, monocytes and CD1c+ blood DCs, highlighting APC specialization in response to vaccines. Finally, the blood signatures from individuals vaccinated with Fluzone or infected with influenza reveal a signature of adaptive immunity activation following vaccination and symptomatic infections, but not asymptomatic infections. These data, offered with a web interface, may guide the development of improved vaccines.

Original languageEnglish (US)
Article number5283
JournalNature communications
Volume5
DOIs
StatePublished - Oct 22 2014

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Banchereau, R., Baldwin, N., Cepika, A. M., Athale, S., Xue, Y., Yu, C. I., Metang, P., Cheruku, A., Berthier, I., Gayet, I., Wang, Y., Ohouo, M., Snipes, L. A., Xu, H., Obermoser, G., Blankenship, D., Oh, S., Ramilo, O., Chaussabel, D., ... Pascual, V. (2014). Transcriptional specialization of human dendritic cell subsets in response to microbial vaccines. Nature communications, 5, [5283]. https://doi.org/10.1038/ncomms6283