Transcriptional analyses of Barrett's metaplasia and normal upper GI mucosae

Michael Barrett, Ka Yee Yeung, Walter L. Ruzzo, Li Hsu, Patricia L. Blount, Robert Sullivan, Helmut Zarbl, Jeffrey Delrow, Peter S. Rabinovitch, Brian J. Reid

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Over the last two decades, the incidence of esophageal adenocarcinoma (EA) has increased dramatically in the US and Western Europe. It has been shown that EAs evolve from premalignant Barrett's esophagus (BE) tissue by a process of clonal expansion and evolution. However, the molecular phenotype of the premalignant metaplasia, and its relationship to those of the normal upper gastrointestinal (GI) mucosae, including gastric, duodenal, and squamous epithelium of the esophagus, has not been systematically characterized. Therefore, we used oligonucleotide-based microarrays to characterize gene expression profiles in each of these tissues. The similarity of BE to each of the normal tissues was compared using a series of computational approaches. Our analyses included esophageal squamous epithelium, which is present at the same anatomic site and exposed to similar conditions as Barrett's epithelium, duodenum that shares morphologic similarity to Barrett's epithelium, and adjacent gastric epithelium. There was a clear distinction among the expression profiles of gastric, duodenal, and squamous epithelium whereas the BE profiles showed considerable overlap with normal tissues. Furthermore, we identified clusters of genes that are specific to each of the tissues, to the Barrett's metaplastic epithelia, and a cluster of genes that was distinct between squamous and nonsquamous epithelia.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalNeoplasia
Volume4
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Barrett Esophagus
Mucous Membrane
Epithelium
Multigene Family
Stomach
Clonal Evolution
Metaplasia
Gastric Mucosa
Oligonucleotide Array Sequence Analysis
Duodenum
Transcriptome
Esophagus
Adenocarcinoma
Phenotype
Incidence

Keywords

  • Barrett's esophagus
  • Clustering
  • Expression
  • Microarray
  • Premalignant

ASJC Scopus subject areas

  • Cancer Research

Cite this

Barrett, M., Yeung, K. Y., Ruzzo, W. L., Hsu, L., Blount, P. L., Sullivan, R., ... Reid, B. J. (2002). Transcriptional analyses of Barrett's metaplasia and normal upper GI mucosae. Neoplasia, 4(2), 121-128. https://doi.org/10.1038/sj/neo/7900221

Transcriptional analyses of Barrett's metaplasia and normal upper GI mucosae. / Barrett, Michael; Yeung, Ka Yee; Ruzzo, Walter L.; Hsu, Li; Blount, Patricia L.; Sullivan, Robert; Zarbl, Helmut; Delrow, Jeffrey; Rabinovitch, Peter S.; Reid, Brian J.

In: Neoplasia, Vol. 4, No. 2, 2002, p. 121-128.

Research output: Contribution to journalArticle

Barrett, M, Yeung, KY, Ruzzo, WL, Hsu, L, Blount, PL, Sullivan, R, Zarbl, H, Delrow, J, Rabinovitch, PS & Reid, BJ 2002, 'Transcriptional analyses of Barrett's metaplasia and normal upper GI mucosae', Neoplasia, vol. 4, no. 2, pp. 121-128. https://doi.org/10.1038/sj/neo/7900221
Barrett, Michael ; Yeung, Ka Yee ; Ruzzo, Walter L. ; Hsu, Li ; Blount, Patricia L. ; Sullivan, Robert ; Zarbl, Helmut ; Delrow, Jeffrey ; Rabinovitch, Peter S. ; Reid, Brian J. / Transcriptional analyses of Barrett's metaplasia and normal upper GI mucosae. In: Neoplasia. 2002 ; Vol. 4, No. 2. pp. 121-128.
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