A transgenic reporter mouse strain, which expressed the human CD25 (hCD25) surface marker as a reporter under the control of the pre-T cell receptor α (pTα) promoter, was used to identify lymphoid precursors that expressed pTα intracellularly. The hCD25 reporter marked intra- and extrathymic precursors of lymphocytes but not myeloid cells. The earliest intrathymic precursors were the extrathymic precursors were common lymphoid progenitors (CLPs) that included CD19−, B220+, Thyl+ and CD4+ cells. Thus, the pTα reporter can be used to trace lymphopoiesis between CLPs and αβ T cells. The slower extinction of the hCD25 reporter compared to pTα enabled us to define points at which pTα− lineages branched off.
|Original language||English (US)|
|Number of pages||8|
|State||Published - 2002|
ASJC Scopus subject areas
- Immunology and Allergy