Time lag of glucose from intravascular to interstitial compartment in humans

Ananda Basu, Simmi Dube, Michael Slama, Isabel Errazuriz, Jose Carlos Amezcua, Yogish C. Kudva, Thomas Peyser, Rickey E. Carter, Claudio Cobelli, Rita Basu

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

The accuracy of continuous interstitial fluid (ISF) glucose sensing is an essential component of current and emerging open-and closed-loop systems for type 1 diabetes. An important determinant of sensor accuracy is the physiological time lag of glucose transport from the vascular to the interstitial space. We performed the first direct measurement of this phenomenon to our knowledge in eight healthy subjects under an overnight fasted condition. Microdialysis catheters were inserted into the abdominal subcutaneous space. After intravenous bolus administrations of glucose tracers, timed samples of plasma and ISF were collected sequentially and analyzed for tracer enrichments. After accounting for catheter dead space and assay noise, the mean time lag of tracer appearance in the interstitial space was 5.3-6.2 min. We conclude that in the overnight fasted state in healthy adults, the physiological delay of glucose transport from the vascular to the interstitial space is 5-6 min. Physiological delay between blood glucose and ISF glucose, therefore, should not be an obstacle to sensor accuracy in overnight or fasting-state closedloop systems of insulin delivery or open-loop therapy assessment for type 1 diabetes.

Original languageEnglish (US)
Pages (from-to)4083-4087
Number of pages5
JournalDiabetes
Volume62
Issue number12
DOIs
StatePublished - Dec 2013

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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    Basu, A., Dube, S., Slama, M., Errazuriz, I., Amezcua, J. C., Kudva, Y. C., Peyser, T., Carter, R. E., Cobelli, C., & Basu, R. (2013). Time lag of glucose from intravascular to interstitial compartment in humans. Diabetes, 62(12), 4083-4087. https://doi.org/10.2337/db13-1132