TY - JOUR
T1 - TIEG1-null osteocytes display defects in their morphology, density and surrounding bone matrix
AU - Haddad, Oualid
AU - Hawse, John R.
AU - Subramaniam, Malayannan
AU - Spelsberg, Thomas C.
AU - Bensamoun, Sabine F.
N1 - Funding Information:
We would like to thank Trace Christensen for his helpful discussions and comments as well as for his assistance with the transmission electron microscopy acquisition. This work was in part supported by an NIH grant DE14036 (TCS).
PY - 2009/9
Y1 - 2009/9
N2 - Through the development of TGFβ-inducible early gene-1 (TIEG1) knockout (KO) mice, we have demonstrated that TIEG1 plays an important role in osteoblast-mediated bone mineralization, and in bone resistance to mechanical strain. To further investigate the influence of TIEG1 in skeletal maintenance, osteocytes were analyzed by transmission electron microscopy using TIEG1 KO and wild-type mouse femurs at one, three and eight months of age. The results revealed an age-dependent change in osteocyte surface and density, suggesting a role for TIEG1 in osteocyte development. Moreover, there was a decrease in the amount of hypomineralized bone matrix surrounding the osteocytes in TIEG1 KO mice relative to wild-type controls. While little is known about the function or importance of this hypomineralized bone matrix immediately adjacent to osteocytes, this study reveals significant differences in this bone microenvironment and suggests that osteocyte function may be compromised in the absence of TIEG1 expression.
AB - Through the development of TGFβ-inducible early gene-1 (TIEG1) knockout (KO) mice, we have demonstrated that TIEG1 plays an important role in osteoblast-mediated bone mineralization, and in bone resistance to mechanical strain. To further investigate the influence of TIEG1 in skeletal maintenance, osteocytes were analyzed by transmission electron microscopy using TIEG1 KO and wild-type mouse femurs at one, three and eight months of age. The results revealed an age-dependent change in osteocyte surface and density, suggesting a role for TIEG1 in osteocyte development. Moreover, there was a decrease in the amount of hypomineralized bone matrix surrounding the osteocytes in TIEG1 KO mice relative to wild-type controls. While little is known about the function or importance of this hypomineralized bone matrix immediately adjacent to osteocytes, this study reveals significant differences in this bone microenvironment and suggests that osteocyte function may be compromised in the absence of TIEG1 expression.
KW - Bone remodeling
KW - Cell morphology
KW - Osteocyte
KW - Transmission Electron Microscopy
UR - http://www.scopus.com/inward/record.url?scp=76449122151&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76449122151&partnerID=8YFLogxK
U2 - 10.1142/S0218957709002304
DO - 10.1142/S0218957709002304
M3 - Article
AN - SCOPUS:76449122151
SN - 0218-9577
VL - 12
SP - 127
EP - 136
JO - Journal of Musculoskeletal Research
JF - Journal of Musculoskeletal Research
IS - 3
ER -