TIEG1-null osteocytes display defects in their morphology, density and surrounding bone matrix

Oualid Haddad, John R. Hawse, Malayannan Subramaniam, Thomas C. Spelsberg, Sabine F. Bensamoun

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Through the development of TGFβ-inducible early gene-1 (TIEG1) knockout (KO) mice, we have demonstrated that TIEG1 plays an important role in osteoblast-mediated bone mineralization, and in bone resistance to mechanical strain. To further investigate the influence of TIEG1 in skeletal maintenance, osteocytes were analyzed by transmission electron microscopy using TIEG1 KO and wild-type mouse femurs at one, three and eight months of age. The results revealed an age-dependent change in osteocyte surface and density, suggesting a role for TIEG1 in osteocyte development. Moreover, there was a decrease in the amount of hypomineralized bone matrix surrounding the osteocytes in TIEG1 KO mice relative to wild-type controls. While little is known about the function or importance of this hypomineralized bone matrix immediately adjacent to osteocytes, this study reveals significant differences in this bone microenvironment and suggests that osteocyte function may be compromised in the absence of TIEG1 expression.

Original languageEnglish (US)
Pages (from-to)127-136
Number of pages10
JournalJournal of Musculoskeletal Research
Volume12
Issue number3
DOIs
StatePublished - Sep 2009

Keywords

  • Bone remodeling
  • Cell morphology
  • Osteocyte
  • Transmission Electron Microscopy

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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