TIEG and estrogen modulate SOST expression in the murine skeleton

Malayannan Subramaniam, Kevin S. Pitel, Elizabeth S. Bruinsma, David G Monroe, John R Hawse

Research output: Contribution to journalArticle

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Abstract

TIEG knockout (KO) mice exhibit a female-specific osteopenic phenotype and altered expression of TIEG in humans is associated with osteoporosis. Gene expression profiling studies identified sclerostin as one of the most highly up-regulated transcripts in the long bones of TIEG KO mice relative to WT littermates suggesting that TIEG may regulate SOST expression. TIEG was shown to substantially suppress SOST promoter activity and the regulatory elements through which TIEG functions were identified using promoter deletion and chromatin immunoprecipitation assays. Knockdown of TIEG in IDG-SW3 osteocyte cells using shRNA and CRISPR-Cas9 technology resulted in increased SOST expression and delayed mineralization, mimicking the results obtained from TIEG KO mouse bones. Given that TIEG is an estrogen regulated gene, and as changes in the hormonal milieu affect SOST expression, we performed ovariectomy (OVX) and estrogen replacement therapy (ERT) studies in WT and TIEG KO mice followed by miRNA and mRNA sequencing of cortical and trabecular compartments of femurs. SOST expression levels were considerably higher in cortical bone compared to trabecular bone. In cortical bone, SOST expression was increased following OVX only in WT mice and was suppressed following ERT in both genotypes. In contrast, SOST expression in trabecular bone was decreased following OVX and significantly increased following ERT. Interestingly, a number of miRNAs that are predicted to target sclerostin exhibited inverse expression levels in response to OVX and ERT. These data implicate important roles for TIEG and estrogen-regulated miRNAs in modulating SOST expression in bone.

Original languageEnglish (US)
Pages (from-to)3540-3551
Number of pages12
JournalJournal of Cellular Physiology
Volume233
Issue number4
DOIs
StatePublished - Apr 1 2018

Fingerprint

Estrogen Replacement Therapy
Skeleton
Knockout Mice
Bone
Estrogens
MicroRNAs
Bone and Bones
Clustered Regularly Interspaced Short Palindromic Repeats
Osteocytes
Chromatin Immunoprecipitation
Ovariectomy
Gene Expression Profiling
Femur
Small Interfering RNA
Osteoporosis
Genotype
Technology
Phenotype
Messenger RNA
Gene expression

Keywords

  • bone
  • estrogen
  • KLF10
  • sclerostin
  • SOST
  • TIEG

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

TIEG and estrogen modulate SOST expression in the murine skeleton. / Subramaniam, Malayannan; Pitel, Kevin S.; Bruinsma, Elizabeth S.; Monroe, David G; Hawse, John R.

In: Journal of Cellular Physiology, Vol. 233, No. 4, 01.04.2018, p. 3540-3551.

Research output: Contribution to journalArticle

Subramaniam, Malayannan ; Pitel, Kevin S. ; Bruinsma, Elizabeth S. ; Monroe, David G ; Hawse, John R. / TIEG and estrogen modulate SOST expression in the murine skeleton. In: Journal of Cellular Physiology. 2018 ; Vol. 233, No. 4. pp. 3540-3551.
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