Thyrotropin receptor antisera for the detection of immunoreactive protein species in retroocular fibroblasts obtained from patients with Graves’ ophthalmopathy

Henry B. Burch, Donald Sellitti, Susan G. Barnes, Endre V. Nagyt, Rebecca S. Bahn, Kenneth D. Burman

Research output: Contribution to journalArticle

Abstract

Autoimmunity against the TSH receptor (hTSH-R) is known to be the proximate cause of thyroidal activation in Graves' disease, but has not been definitively linked to extrathyroidal manifestations of this disorder, such as ophthalmopathy and pretibial myxedema. In an effort to increase our knowledge concerning mechanisms responsible for Graves' ophthalmopathy, we used antiserum directed against a highly immunogenic portion of the hTSH-R (amino acids 352-367; P1) to assess the presence of this receptor or immunologically related protein in cultured human retroocular fibroblasts obtained from patients with Graves' ophthalmopathy. Immunoenzymatic and immunofluorescent studies revealed specific staining of both cytoplasmic and cell membrane-associated protein in discrete vesicles. To further evaluate the immunoreactive species present in these cells, immunoblotting experiments were performed using hTSH-R-specific antisera (anti-P1) and sera obtained from patients with Graves' disease. Several protein bands were identified using both anti-P1 and Graves' disease patient sera, including species at mol wt of 95, 71, and 18 kilodaltons, the possible significance of which is discussed. The results support the hypothesis that immunity against the hTSH-R or related proteins contributes to the ophthalmopathy of Graves' disease.

Original languageEnglish (US)
Pages (from-to)1384-1391
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume78
Issue number6
DOIs
StatePublished - 1994

Fingerprint

Graves Ophthalmopathy
Thyrotropin Receptors
Graves Disease
Fibroblasts
Immune Sera
Membrane Proteins
Proteins
Myxedema
Cell membranes
Serum
Autoimmunity
Immunoblotting
Immunity
Chemical activation
Cell Membrane
Staining and Labeling
Amino Acids
Experiments

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Thyrotropin receptor antisera for the detection of immunoreactive protein species in retroocular fibroblasts obtained from patients with Graves’ ophthalmopathy. / Burch, Henry B.; Sellitti, Donald; Barnes, Susan G.; Nagyt, Endre V.; Bahn, Rebecca S.; Burman, Kenneth D.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 78, No. 6, 1994, p. 1384-1391.

Research output: Contribution to journalArticle

Burch, Henry B. ; Sellitti, Donald ; Barnes, Susan G. ; Nagyt, Endre V. ; Bahn, Rebecca S. ; Burman, Kenneth D. / Thyrotropin receptor antisera for the detection of immunoreactive protein species in retroocular fibroblasts obtained from patients with Graves’ ophthalmopathy. In: Journal of Clinical Endocrinology and Metabolism. 1994 ; Vol. 78, No. 6. pp. 1384-1391.
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