Three-dimensional porous poly(propylene fumarate)-co-poly(lactic-co-glycolic acid) scaffolds for tissue engineering

Wei Wu, Xifeng Liu, Zifei Zhou, A. Lee Miller, Lichun Lu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Three-dimensional structural scaffolds have played an important role in tissue engineering, especially broad applications in areas such as regenerative medicine. We have developed novel biodegradable porous poly(propylene fumarate)-co-poly(lactic-co-glycolic acid) (PPF-co-PLGA) scaffolds using thermally induced phase separation, and determined the effects of critical parameters such as copolymer concentration (6, 8, and 10 wt %) and the binary solvent ratio of dioxane:water (78/22, 80/20, 82/18 wt/wt %) on the fabrication process. The cloud-point temperatures of PPF-co-PLGA changed in parallel with increasing copolymer concentration, but inversely with increasing dioxane content. The compressive moduli of the scaffolds increased with greater weight composition and dioxane:water ratio. Scaffolds formed using high copolymer concentrations and solvent ratios exhibited preferable biomineralization. All samples showed biodegradation capability in both accelerated solution and phosphate-buffered saline (PBS). Cell toxicity testing indicated that the scaffolds had good biocompatibility with bone and nerve cells, which adhered well to the scaffolds. Variations in the copolymer concentration and solvent ratio exercised a remarkable influence on morphology, mechanical properties, biomineralization, and biodegradation, but not on the cell viability and adhesion of the cross-linked scaffolds. An 8 to 10 wt % solute concentration and 80/20 to 82/18 wt/wt dioxane:water ratio were the optimum parameters for scaffold fabrication. PPF-co-PLGA scaffolds thus possess several promising prospects for tissue engineering applications.

Original languageEnglish (US)
Pages (from-to)2507-2517
Number of pages11
JournalJournal of Biomedical Materials Research - Part A
Volume106
Issue number9
DOIs
StatePublished - Sep 1 2018
Externally publishedYes

Fingerprint

Scaffolds (biology)
Tissue engineering
Scaffolds
Polypropylenes
Acids
Copolymers
Biomineralization
Biodegradation
Water
Fabrication
Biocompatibility
Phase separation
Neurons
Toxicity
Milk
poly(propylene fumarate)
polylactic acid-polyglycolic acid copolymer
Bone
Phosphates
Adhesion

Keywords

  • biomineralization
  • interconnected porosity
  • phase separation
  • three-dimensional scaffolds
  • tissue engineering

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Three-dimensional porous poly(propylene fumarate)-co-poly(lactic-co-glycolic acid) scaffolds for tissue engineering. / Wu, Wei; Liu, Xifeng; Zhou, Zifei; Miller, A. Lee; Lu, Lichun.

In: Journal of Biomedical Materials Research - Part A, Vol. 106, No. 9, 01.09.2018, p. 2507-2517.

Research output: Contribution to journalArticle

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abstract = "Three-dimensional structural scaffolds have played an important role in tissue engineering, especially broad applications in areas such as regenerative medicine. We have developed novel biodegradable porous poly(propylene fumarate)-co-poly(lactic-co-glycolic acid) (PPF-co-PLGA) scaffolds using thermally induced phase separation, and determined the effects of critical parameters such as copolymer concentration (6, 8, and 10 wt {\%}) and the binary solvent ratio of dioxane:water (78/22, 80/20, 82/18 wt/wt {\%}) on the fabrication process. The cloud-point temperatures of PPF-co-PLGA changed in parallel with increasing copolymer concentration, but inversely with increasing dioxane content. The compressive moduli of the scaffolds increased with greater weight composition and dioxane:water ratio. Scaffolds formed using high copolymer concentrations and solvent ratios exhibited preferable biomineralization. All samples showed biodegradation capability in both accelerated solution and phosphate-buffered saline (PBS). Cell toxicity testing indicated that the scaffolds had good biocompatibility with bone and nerve cells, which adhered well to the scaffolds. Variations in the copolymer concentration and solvent ratio exercised a remarkable influence on morphology, mechanical properties, biomineralization, and biodegradation, but not on the cell viability and adhesion of the cross-linked scaffolds. An 8 to 10 wt {\%} solute concentration and 80/20 to 82/18 wt/wt dioxane:water ratio were the optimum parameters for scaffold fabrication. PPF-co-PLGA scaffolds thus possess several promising prospects for tissue engineering applications.",
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