The transcription factors Scl and Lmo2 act together during development of the hemangioblast in zebrafish

Lucy J. Patterson, Martin Gering, Craig E. Eckfeldt, Anthony R. Green, Catherine M. Verfaillie, Stephen C Ekker, Roger Patient

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The transcription factors Scl and Lmo2 are crucial for development of all blood. An important early requirement for Scl in endothelial development has also been revealed recently in zebrafish embryos, supporting previous findings in scl-/- embryoid bodies. Scl depletion culminates most notably in failure of dorsal aorta formation, potentially revealing a role in the formation of hemogenic endothelium. We now present evidence that the requirements for Lmo2 in zebrafish embryos are essentially the same as for Scl. The expression of important hematopoietic regulators is lost, reduced, or delayed, panendothelial gene expression is down-regulated, and aorta-specific marker expression is lost. The close similarity of the phenotypes for Scl and Lmo2 suggest that they perform these early functions in hemangioblast development within a multiprotein complex, as shown for erythropoiesis. Consistent with this, we find that scl morphants cannot be rescued by a non-Lmo2-binding form of Scl but can be rescued by non-DNA-binding forms, suggesting tethering to target genes through DNA-binding partners linked via Lmo2. Interestingly, unlike other hematopoietic regulators, the Scl/Lmo2 complex does not appear to autoregulate, as neither gene's expression is affected by depletion of the other. Thus, expression of these critical regulators is dependent on continued expression of upstream regulators, which may include cell-extrinsic signals.

Original languageEnglish (US)
Pages (from-to)2389-2398
Number of pages10
JournalBlood
Volume109
Issue number6
DOIs
StatePublished - Mar 15 2007
Externally publishedYes

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Hemangioblasts
Zebrafish
Gene expression
Aorta
Transcription Factors
Embryonic Structures
Embryoid Bodies
Gene Expression
Multiprotein Complexes
Erythropoiesis
Blood
Genes
Phenotype
DNA

ASJC Scopus subject areas

  • Hematology

Cite this

Patterson, L. J., Gering, M., Eckfeldt, C. E., Green, A. R., Verfaillie, C. M., Ekker, S. C., & Patient, R. (2007). The transcription factors Scl and Lmo2 act together during development of the hemangioblast in zebrafish. Blood, 109(6), 2389-2398. https://doi.org/10.1182/blood-2006-02-003087

The transcription factors Scl and Lmo2 act together during development of the hemangioblast in zebrafish. / Patterson, Lucy J.; Gering, Martin; Eckfeldt, Craig E.; Green, Anthony R.; Verfaillie, Catherine M.; Ekker, Stephen C; Patient, Roger.

In: Blood, Vol. 109, No. 6, 15.03.2007, p. 2389-2398.

Research output: Contribution to journalArticle

Patterson, LJ, Gering, M, Eckfeldt, CE, Green, AR, Verfaillie, CM, Ekker, SC & Patient, R 2007, 'The transcription factors Scl and Lmo2 act together during development of the hemangioblast in zebrafish', Blood, vol. 109, no. 6, pp. 2389-2398. https://doi.org/10.1182/blood-2006-02-003087
Patterson, Lucy J. ; Gering, Martin ; Eckfeldt, Craig E. ; Green, Anthony R. ; Verfaillie, Catherine M. ; Ekker, Stephen C ; Patient, Roger. / The transcription factors Scl and Lmo2 act together during development of the hemangioblast in zebrafish. In: Blood. 2007 ; Vol. 109, No. 6. pp. 2389-2398.
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