The specificity and significance of the inhibition of Fc receptor binding by anti H 2 sera

P. Halloran, V. Schirrmacher, C. S. David

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The possibility that Ia antigens are unique among H 2 antigens in their relationship to the Fc receptor was investigated in an EA rosette assay. Antibody specific for antigens in various regions of the H 2 complex was incubated with mouse cells, and the ability of the cells to form rosettes with antibody coated chicken erythrocytes was tested. Antibody raised against the H 2 antigens of Ia negative tumor cells was highly effective in inhibiting rosette formation. A variety of antisera against K, I, and D region antigens tested in recombinant mice inhibited EA rosette formation, suggesting that antigens in each of these regions could be detected in rosette inhibition. The F(ab') 2 fragments of all antisera tested also produced specific EA rosette inhibition. Finally, antibody against Ia antigens failed to inhibit bone marrow RFCs, although antibody against H 2K and H 2D antigens did inhibit. Although H 2 serology is in a state of rapid change at present, it must be concluded that in this assay, antibody against antigens in the K and D regions as well as the I region can inhibit EA rosette formation. Inhibition of these rosettes by anti H 2 sera is therefore not due to a special association of Ia antigens with Fc receptors.

Original languageEnglish (US)
Pages (from-to)349-361
Number of pages13
JournalImmunogenetics
Volume2
Issue number4
StatePublished - 1975
Externally publishedYes

Fingerprint

Fc Receptors
Rosette Formation
Antibodies
Histocompatibility Antigens Class II
Serum
H-2 Antigens
Antigens
Immune Sera
Serology
Chickens
Erythrocytes
Bone Marrow
Neoplasms

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Halloran, P., Schirrmacher, V., & David, C. S. (1975). The specificity and significance of the inhibition of Fc receptor binding by anti H 2 sera. Immunogenetics, 2(4), 349-361.

The specificity and significance of the inhibition of Fc receptor binding by anti H 2 sera. / Halloran, P.; Schirrmacher, V.; David, C. S.

In: Immunogenetics, Vol. 2, No. 4, 1975, p. 349-361.

Research output: Contribution to journalArticle

Halloran, P, Schirrmacher, V & David, CS 1975, 'The specificity and significance of the inhibition of Fc receptor binding by anti H 2 sera', Immunogenetics, vol. 2, no. 4, pp. 349-361.
Halloran, P. ; Schirrmacher, V. ; David, C. S. / The specificity and significance of the inhibition of Fc receptor binding by anti H 2 sera. In: Immunogenetics. 1975 ; Vol. 2, No. 4. pp. 349-361.
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