The sequential alterations of endoneurial cholesterol and fatty acid in Wallerian degeneration and regeneration

J. K. Yao, V. Natarajan, Peter J Dyck

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The endoneurial nonpolar lipids were serially examined throughout Wallerian degeneration and regeneration. Following nerve crush in the rat, the endoneurial content of cholesterol falls and cholesteryl ester content rises dramatically. The maximal alteration of this ratio corresponds reasonably well with events of myelin ovoid dissolution to sudanophilic amorphous lipids. Thereafter, as regenerative events overshadow degenerative events the ratio is slowly restored toward normal. The increased cholesterol esters are probably synthesized within endoneurium from free fatty acids which become available when myelin is degraded. The endoneurial free fatty acid content presumably represents the net effect of phospholipid degradation, cholesterol esterification, cholesteryl ester hydrolysis, and fasciculr entry and exit. Free fatty acids become significantly elevated by 12 days, probably reach a peak between 16 and 60 days, and thereafter return to normal with fiber regeneration. The fatty acid composition of cholesteryl esters from crushed nerves is markedly differrent from those of normal sciatic nerves. The altered fatty acid composition of cholesteryl esters from myelin suggests that both synthesis and hydrolysis exhibit substrate specificity toward chain length and unsaturaton, with oleate being the most favored substrate.

Original languageEnglish (US)
Pages (from-to)933-940
Number of pages8
JournalJournal of Neurochemistry
Volume35
Issue number4
StatePublished - 1980

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Wallerian Degeneration
Cholesterol Esters
Regeneration
Fatty Acids
Cholesterol
Nonesterified Fatty Acids
Myelin Sheath
Hydrolysis
Lipids
Nerve Crush
Esterification
Substrates
Oleic Acid
Chemical analysis
Chain length
Sciatic Nerve
Rats
Substrate Specificity
Phospholipids
Peripheral Nerves

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

The sequential alterations of endoneurial cholesterol and fatty acid in Wallerian degeneration and regeneration. / Yao, J. K.; Natarajan, V.; Dyck, Peter J.

In: Journal of Neurochemistry, Vol. 35, No. 4, 1980, p. 933-940.

Research output: Contribution to journalArticle

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