TY - JOUR
T1 - The role of reverse genetics systems in studying viral hemorrhagic fevers
AU - Ebihara, Hideki
AU - Groseth, Allison
AU - Neumann, Gabriele
AU - Kawaoka, Yoshihiro
AU - Feldmann, Heinz
PY - 2005/8
Y1 - 2005/8
N2 - Viral hemorrhagic fever (VHF) is an infectious syndrome in humans often associated with high fatality rates. For most VHFs there are no specific and effective therapies or vaccines available and, in general, there is a lack of knowledge regarding the biology and pathogenesis of the causative agents. Therefore, a more detailed understanding of the molecular basis of VHF pathogenesis, including the identification of viral virulence determinants and host interactions and responses, will be important to enhance our ability to control VHF infections. The recently developed "reverse genetics systems" for several VHF causing viruses have allowed the generation of infectious viruses from cloned cDNA and thus, the generation of virus mutants. Here we review the existing reverse genetics systems for VHF causing viruses and discuss their use in studying viral replication, pathogenesis, and the development of antivirals and vaccines.
AB - Viral hemorrhagic fever (VHF) is an infectious syndrome in humans often associated with high fatality rates. For most VHFs there are no specific and effective therapies or vaccines available and, in general, there is a lack of knowledge regarding the biology and pathogenesis of the causative agents. Therefore, a more detailed understanding of the molecular basis of VHF pathogenesis, including the identification of viral virulence determinants and host interactions and responses, will be important to enhance our ability to control VHF infections. The recently developed "reverse genetics systems" for several VHF causing viruses have allowed the generation of infectious viruses from cloned cDNA and thus, the generation of virus mutants. Here we review the existing reverse genetics systems for VHF causing viruses and discuss their use in studying viral replication, pathogenesis, and the development of antivirals and vaccines.
KW - Pathogenesis
KW - Reverse genetic systems
KW - Viral hemorrhagic fever
UR - http://www.scopus.com/inward/record.url?scp=23744512867&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23744512867&partnerID=8YFLogxK
U2 - 10.1160/TH05-05-0335
DO - 10.1160/TH05-05-0335
M3 - Review article
C2 - 16113812
AN - SCOPUS:23744512867
SN - 0340-6245
VL - 94
SP - 240
EP - 253
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
IS - 2
ER -