The role of purinergic signaling on deformation induced injury and repair responses of alveolar epithelial cells

Hewan A. Belete, Rolf D. Hubmayr, Shaohua Wang, Raman Deep Singh

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Cell wounding is an important driver of the innate immune response of ventilator-injured lungs. We had previously shown that the majority of wounded alveolus resident cells repair and survive deformation induced insults. This is important insofar as wounded and repaired cells may contribute to injurious deformation responses commonly referred to as biotrauma. The central hypothesis of this communication states that extracellular adenosine-5′ triphosphate (ATP) promotes the repair of wounded alveolus resident cells by a P2Y2-Receptor dependent mechanism. Using primary type 1 alveolar epithelial rat cell models subjected to micropuncture injury and/or deforming stress we show that 1) stretch causes a dose dependent increase in cell injury and ATP media concentrations; 2) enzymatic depletion of extracellular ATP reduces the probability of stretch induced wound repair; 3) enriching extracellular ATP concentrations facilitates wound repair; 4) purinergic effects on cell repair are mediated by ATP and not by one of its metabolites; and 5) ATP mediated cell salvage depends at least in part on P2Y2-R activation. While rescuing cells from wounding induced death may seem appealing, it is possible that survivors of membrane wounding become governors of a sustained pro-inflammatory state and thereby perpetuate and worsen organ function in the early stages of lung injury syndromes. Means to uncouple P2Y2-R mediated cytoprotection from P2Y2-R mediated inflammation and to test the preclinical efficacy of such an undertaking deserve to be explored.

Original languageEnglish (US)
Article numbere27469
JournalPLoS One
Volume6
Issue number11
DOIs
StatePublished - Nov 8 2011

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Alveolar Epithelial Cells
Adenosine
epithelial cells
Repair
adenosine triphosphate
Adenosine Triphosphate
Wounds and Injuries
cells
Purinergic Agents
Purinergic P2Y2 Receptors
Salvaging
animal injuries
Governors
Metabolites
lungs
Cytoprotection
triphosphoric acid
Epithelial Cells
Rats
ventilators

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The role of purinergic signaling on deformation induced injury and repair responses of alveolar epithelial cells. / Belete, Hewan A.; Hubmayr, Rolf D.; Wang, Shaohua; Singh, Raman Deep.

In: PLoS One, Vol. 6, No. 11, e27469, 08.11.2011.

Research output: Contribution to journalArticle

Belete, Hewan A. ; Hubmayr, Rolf D. ; Wang, Shaohua ; Singh, Raman Deep. / The role of purinergic signaling on deformation induced injury and repair responses of alveolar epithelial cells. In: PLoS One. 2011 ; Vol. 6, No. 11.
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