The microenvironment of human Peyer's patches inhibits the increase in CD38 expression associated with the germinal center reaction

M. J. Guilliano, A. E. Foxx-Orenstein, D. A. Lebman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Analysis of B cells in the human tonsils identified CD38 expression as a hallmark of germinal center (GC) B cells. However, the signals responsible for the in vivo induction of CD38 have not been determined. The primary site for generation of memory and plasma cells in the gastrointestinal tract is the GCs of Peyer's patches (PP). PP and intestinal mucosa, but not tonsils or oral mucosa, express mucosal addressin cell adhesion molecule-1 (MAdCAM-1). The ligand for MAdCAM-1, integrin α4β7, is expressed on naive B cells and memory B cells that traffic to the gastrointestinal tract. In this study we determine that, unlike tonsil, human PP GC B cells do not express significant levels of CD38. PP B cells can be induced to express CD38 upon culture with CD40 ligand, anti-B cell receptor, and IFN-γ. However, coculture of tonsil naive B cells with an Ab directed against integrin β7 inhibits IFN-γ-induced CD38 hyperexpression. The absence of CD38 on PP GCs suggests that there are tissue-specific pathways of B cell development that differ between tonsil and PP. The differential expression pattern of MAdCAM-1, together with the observation that ligation of β7 can inhibit the induction of CD38 expression, suggests that ligation of α4β7 in vivo may contribute to a PP-specific GC phenotype.

Original languageEnglish (US)
Pages (from-to)2179-2185
Number of pages7
JournalJournal of Immunology
Volume166
Issue number4
StatePublished - Feb 15 2001
Externally publishedYes

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Peyer's Patches
Germinal Center
B-Lymphocytes
Palatine Tonsil
Cell Adhesion Molecules
Integrins
Ligation
Gastrointestinal Tract
CD40 Ligand
Mouth Mucosa
Intestinal Mucosa
Coculture Techniques
Plasma Cells
Ligands
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

The microenvironment of human Peyer's patches inhibits the increase in CD38 expression associated with the germinal center reaction. / Guilliano, M. J.; Foxx-Orenstein, A. E.; Lebman, D. A.

In: Journal of Immunology, Vol. 166, No. 4, 15.02.2001, p. 2179-2185.

Research output: Contribution to journalArticle

Guilliano, M. J. ; Foxx-Orenstein, A. E. ; Lebman, D. A. / The microenvironment of human Peyer's patches inhibits the increase in CD38 expression associated with the germinal center reaction. In: Journal of Immunology. 2001 ; Vol. 166, No. 4. pp. 2179-2185.
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