The measles virus hemagglutinin β-propeller head β4- β5 hydrophobic groove governs functional interactions with nectin-4 and CD46 but not those with the signaling lymphocytic activation molecule

Mathieu Mateo, Chanakha K. Navaratnarajah, Sabriya Syed, Roberto Cattaneo

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Wild-type measles virus (MV) strains use the signaling lymphocytic activation molecule (SLAM; CD150) and the adherens junction protein nectin-4 (poliovirus receptor-like 4 [PVRL4]) as receptors. Vaccine MV strains have adapted to use ubiquitous membrane cofactor protein (MCP; CD46) in addition. Recently solved cocrystal structures of the MV attachment protein (hemagglutinin [H]) with each receptor indicate that all three bind close to a hydrophobic groove located between blades 4 and 5 ( MathematicalPi-Four.-1.H9252 4- β5 groove) of the H protein β-propeller head. We used this structural information to focus our analysis of the functional footprints of the three receptors on vaccine MV H. We mutagenized this protein and tested the ability of individual mutants to support cell fusion through each receptor. The results highlighted a strong overlap between the functional footprints of nectin-4 and CD46 but not those of SLAM. A soluble form of nectin-4 abolished vaccine MV entry in nectin-4and CD46-expressing cells but only reduced entry through SLAM. Analyses of the binding kinetics of an H mutant with the three receptors revealed that a single substitution in the β4- β5 groove drastically reduced nectin-4 and CD46 binding while minimally altering SLAM binding. We also generated recombinant viruses and analyzed their infections in cells expressing individual receptors. Introduction of a single substitution into the hydrophobic pocket affected entry through both nectin-4 and CD46 but not through SLAM. Thus, while nectin-4 and CD46 interact functionally with the H protein β4- β5 hydrophobic groove, SLAM merely covers it. This has implications for vaccine and antiviral strategies.

Original languageEnglish (US)
Pages (from-to)9208-9216
Number of pages9
JournalJournal of Virology
Volume87
Issue number16
DOIs
StatePublished - Aug 2013

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Measles virus
Hemagglutinins
hemagglutinins
Head
receptors
Vaccines
vaccines
proteins
Proteins
CD46 Antigens
Adherens Junctions
Virus Attachment
Enterovirus C
Virus Internalization
mutants
cell fusion
Cell Fusion
Signaling Lymphocytic Activation Molecule Family
nectins
Antiviral Agents

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

The measles virus hemagglutinin β-propeller head β4- β5 hydrophobic groove governs functional interactions with nectin-4 and CD46 but not those with the signaling lymphocytic activation molecule. / Mateo, Mathieu; Navaratnarajah, Chanakha K.; Syed, Sabriya; Cattaneo, Roberto.

In: Journal of Virology, Vol. 87, No. 16, 08.2013, p. 9208-9216.

Research output: Contribution to journalArticle

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