The impact of eculizumab on routine complement assays

Maria A.V. Willrich, Bruna D. Andreguetto, Meera Sridharan, Fernando Custodio Fervenza, Linda J. Tostrud, Paula M. Ladwig, Ann M. Rivard, Me Lea D. Hetrick, Ryan N. Olson, Sandra C. Bryant, Melissa R. Snyder, David L. Murray

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6 Scopus citations

Abstract

Background: Eculizumab (ECU) blocks complement C5 cleavage, preventing the formation of C5a and the cytolytic effects of the membrane attack complex. The presence of ECU in blood impacts routine complement tests used to monitor treatment. Methods: Residual serum samples with normal total complement (CH50) and residual citrate plasma with normal PT/APTT were spiked with ECU at varied concentrations ranging from 25 to 600 μg/mL. In addition, seventy-one samples from patients on ECU were obtained. Artificial and patient samples were analyzed for CH50 and C5 function (Wako Diagnostics), C5 concentration (Quidel), AH50 (Wieslab ELISA) and sMAC (Quidel). ECU concentration was measured by mass spectrometry for all patients. Results: Complement blockage by ECU was evident in spiked artificial samples. At 25 μg/mL ECU, partial complement blockage was observed in CH50, AH50 and C5 function in serum. Complete blockage defined by undetectable AH50 (<10%) occurred at 100 μg/mL ECU. C5 concentrations remained the same regardless of ECU. sMAC results stayed around 81% of baseline in serum and 47% in citrate plasma with 50μg/mL ECU. Patient samples had ECU ranging from <5 to 1220 μg/mL. In all patients with ECU >100 μg/mL, C5 function was <29 U/mL. Conclusions: The spiked sera and patient samples showed complement blockage with CH50, AH50 and C5 function assays when ECU >100 μg/mL. CH50, AH50 or C5 function assays can serve as indicators for the pharmacodynamic effects of eculizumab. Allied to ECU concentration, laboratory studies may be helpful to tailor therapy.

Original languageEnglish (US)
JournalJournal of Immunological Methods
DOIs
StateAccepted/In press - Jan 1 2018

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Keywords

  • Alternative pathway
  • C5 inhibitor
  • Complement
  • Complement C5 component
  • Eculizumab
  • Soluble membrane attack complex

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Willrich, M. A. V., Andreguetto, B. D., Sridharan, M., Fervenza, F. C., Tostrud, L. J., Ladwig, P. M., Rivard, A. M., Hetrick, M. L. D., Olson, R. N., Bryant, S. C., Snyder, M. R., & Murray, D. L. (Accepted/In press). The impact of eculizumab on routine complement assays. Journal of Immunological Methods. https://doi.org/10.1016/j.jim.2018.06.010