The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR

Yasushi Kawaguchi; Ken Maeda; Marcelo R. Pecoraro; Yasuo Inoshima; Hyung kwan Jang; Mariko Kohmoto; Kiyoko Iwatsuki; Yasuhiro Ikeda; Masahiro Shimojima; Yukinobu Tohya; Chieko Kai; Takeshi Mikami

doi:10.1292/jvms.57.1129

The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR

Yasushi Kawaguchi, Ken Maeda, Marcelo R. Pecoraro, Yasuo Inoshima, Hyung kwan Jang, Mariko Kohmoto, Kiyoko Iwatsuki, Yasuhiro Ikeda, Masahiro Shimojima, Yukinobu Tohya, Chieko Kai, Takeshi Mikami

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.

Original language	English (US)
Pages (from-to)	1129-1131
Number of pages	3
Journal	Journal of Veterinary Medical Science
Volume	57
Issue number	6
DOIs	https://doi.org/10.1292/jvms.57.1129
State	Published - 1995

Keywords

C/EBP site
FHV-1 ICP4
FIV LTR

ASJC Scopus subject areas

General Veterinary

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10.1292/jvms.57.1129

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Kawaguchi, Y., Maeda, K., Pecoraro, M. R., Inoshima, Y., Jang, H. K., Kohmoto, M., Iwatsuki, K., Ikeda, Y., Shimojima, M., Tohya, Y., Kai, C., & Mikami, T. (1995). The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR. Journal of Veterinary Medical Science, 57(6), 1129-1131. https://doi.org/10.1292/jvms.57.1129

The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR. / Kawaguchi, Yasushi; Maeda, Ken; Pecoraro, Marcelo R. et al.
In: Journal of Veterinary Medical Science, Vol. 57, No. 6, 1995, p. 1129-1131.

Research output: Contribution to journal › Article › peer-review

Kawaguchi, Y, Maeda, K, Pecoraro, MR, Inoshima, Y, Jang, HK, Kohmoto, M, Iwatsuki, K, Ikeda, Y, Shimojima, M, Tohya, Y, Kai, C & Mikami, T 1995, 'The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR', Journal of Veterinary Medical Science, vol. 57, no. 6, pp. 1129-1131. https://doi.org/10.1292/jvms.57.1129

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title = "The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR",

abstract = "We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.",

keywords = "C/EBP site, FHV-1 ICP4, FIV LTR",

author = "Yasushi Kawaguchi and Ken Maeda and Pecoraro, {Marcelo R.} and Yasuo Inoshima and Jang, {Hyung kwan} and Mariko Kohmoto and Kiyoko Iwatsuki and Yasuhiro Ikeda and Masahiro Shimojima and Yukinobu Tohya and Chieko Kai and Takeshi Mikami",

year = "1995",

doi = "10.1292/jvms.57.1129",

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AU - Maeda, Ken

AU - Pecoraro, Marcelo R.

AU - Inoshima, Yasuo

AU - Jang, Hyung kwan

AU - Kohmoto, Mariko

AU - Iwatsuki, Kiyoko

AU - Ikeda, Yasuhiro

AU - Shimojima, Masahiro

AU - Tohya, Yukinobu

AU - Kai, Chieko

AU - Mikami, Takeshi

PY - 1995

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N2 - We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.

AB - We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.

KW - C/EBP site

KW - FHV-1 ICP4

KW - FIV LTR

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The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat(LTR)-directed gene expression via the C/EBP site in the LTR

Abstract

Keywords

ASJC Scopus subject areas

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