The effects of polymorphisms on human gene targeting

David R. Deyle; Li B. Li; Gaoying Ren; David W. Russell

doi:10.1093/nar/gkt1303

The effects of polymorphisms on human gene targeting

David R. Deyle, Li B. Li, Gaoying Ren, David W. Russell

Medical Genetics

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting or to improve unfavorable vector designs that introduce deletions.

Original language	English (US)
Pages (from-to)	3119-3124
Number of pages	6
Journal	Nucleic acids research
Volume	42
Issue number	5
DOIs	https://doi.org/10.1093/nar/gkt1303
State	Published - Mar 1 2014

ASJC Scopus subject areas

Genetics

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10.1093/nar/gkt1303

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abstract = "DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting or to improve unfavorable vector designs that introduce deletions.",

author = "Deyle, {David R.} and Li, {Li B.} and Gaoying Ren and Russell, {David W.}",

note = "Funding Information: Funding for open access charge: National Institutes of Health [AR53917 and AR53917-02S1 to D.D.; DK55759, HL53750 and AR48328 to D.R.].",

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AU - Deyle, David R.

AU - Li, Li B.

AU - Ren, Gaoying

AU - Russell, David W.

N1 - Funding Information: Funding for open access charge: National Institutes of Health [AR53917 and AR53917-02S1 to D.D.; DK55759, HL53750 and AR48328 to D.R.].

PY - 2014/3/1

Y1 - 2014/3/1

N2 - DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting or to improve unfavorable vector designs that introduce deletions.

AB - DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In addition, we show that homology arm polymorphisms can be used to direct allele-specific targeting or to improve unfavorable vector designs that introduce deletions.

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The effects of polymorphisms on human gene targeting

Abstract

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