The effect of peroxynitrite on sphincter of Oddi motility

Brian W. Herrmann, Joseph J. Cullen, Amber Ledlow, Joseph A Murray, Jeffrey L. Conklin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background. Nitric oxide (NO*) is an inhibitory neurotransmitter that induces sphincter of Oddi relaxation. Superoxide (O 2/(·-)-scavenging enzymes are present in enteric plexuses of the sphincter of Oddi and O 2/(·- ) alters sphincter of Oddi motor function. O 2/(·-) rapidly oxidizes nitric oxide (NO*) to form peroxynitrite (ONOO -), thus terminating the biological activity of NO*. The aim of our study was to determine the effects of ONOO - on sphincter of Oddi motility in vitro. Materials and methods. Adult opossums were sacrificed and the sphincter of Oddi was removed and placed in a tissue bath containing oxygenated Krebs solution at 37°C. In the first series of experiments, force transducers recorded tension in a transverse orientation at two sites along the spontaneously contracting sphincter of Oddi. In a second series of experiments, circular muscle strips were precontracted with carbachol and stimulated by an electrical field. Results. ONOO -, superoxide dismutase (SOD), Nω-nitro-L-arginine (L-NNA), or oxyhemoglobin were added to the tissue baths. ONOO - decreased the frequency of contractions in the spontaneously contracting sphincter of Oddi. Adding hemoglobin increased the frequency of contractions. ONOO - also increased the stimulation-induced relaxation compared to controls. The increase in relaxation induced by ONOO - was inhibited by oxyhemoglobin and L-NNA but not SOD. Pretreatment with oxyhemoglobin prevented the increase in the stimulation-induced relaxation caused by ONOO -. Conclusion. These results suggest that hemoglobin binds ONOO - or that ONOO - generates NO.

Original languageEnglish (US)
Pages (from-to)55-58
Number of pages4
JournalJournal of Surgical Research
Volume81
Issue number1
DOIs
StatePublished - Jan 1999
Externally publishedYes

Fingerprint

Sphincter of Oddi
Peroxynitrous Acid
Oxyhemoglobins
Nitric Oxide
Baths
Superoxide Dismutase
Hemoglobins
Opossums
Carbachol
Transducers
Superoxides
Neurotransmitter Agents
Arginine
Muscles
Enzymes

Keywords

  • Motility
  • Peroxynitrite
  • Sphincter of Oddi

ASJC Scopus subject areas

  • Surgery

Cite this

The effect of peroxynitrite on sphincter of Oddi motility. / Herrmann, Brian W.; Cullen, Joseph J.; Ledlow, Amber; Murray, Joseph A; Conklin, Jeffrey L.

In: Journal of Surgical Research, Vol. 81, No. 1, 01.1999, p. 55-58.

Research output: Contribution to journalArticle

Herrmann, Brian W. ; Cullen, Joseph J. ; Ledlow, Amber ; Murray, Joseph A ; Conklin, Jeffrey L. / The effect of peroxynitrite on sphincter of Oddi motility. In: Journal of Surgical Research. 1999 ; Vol. 81, No. 1. pp. 55-58.
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