TY - JOUR
T1 - The Effect of Melatonin on Aqueous Humor Flow in Humans during the Day
AU - Viggiano, Suzanne R.
AU - Koskela, Timo K.
AU - Klee, George G.
AU - Samples, John R.
AU - Arnce, Robert
AU - Brubaker, Richard F.
N1 - Funding Information:
Originally received: March 31, 1993. Revision accepted: July 26, 1993. I Mayo Medical School, Rochester, Minnesota. 2 Departments of Ophthalmology, Mayo Clinic, Rochester, Minnesota. 3 Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota. 4 Department of Ophthalmology, Oregon Health Sciences University, Portland. Dr. Viggiano currently is affiliated with the Department of Ophthalmology at the Mayo Clinic. Supported in part by NIH grant EY 00634 (Dr. Brubaker),· Bethesda, Maryland; Clinical Research Center grant RR 585A, Research to Prevent
PY - 1994
Y1 - 1994
N2 - Background: Aqueous humor flow through the anterior chamber of the eye undergoes a circadian cycle. The rate of flow during the day is twice as high as the rate of flow at night. The pineal hormone, melatonin, also undergoes a circadian cycle. Melatonin levels are high at night, whereas aqueous humor flow is low. The authors studied the effect of oral melatonin on aqueous humor flow in humans. Methods: The effect of melatonin on aqueous humor flow was evaluated in 19 healthy human volunteers in a randomized, masked crossover study with a placebo control. The hormone or placebo was administered orally during the day when endogenous levels of melatonin are low. Aqueous flow was measured by fluorophotometry for 8 hours. Results: The mean rate of flow during melatonin treatment was 2.71 ± 0.64 μl/minute (± standard deviation). The rate of flow during placebo treatment was 2.80 ± 0.66 μl/minute. There is no statistically significant difference between these two rates (P = 0.4). With a sample size of 19, the study has a power of 92% to detect at least a 15% difference in the rate of flow under the two conditions. Measurement of plasma concentration of melatonin in five subjects confirmed that concentrations after oral dosage reached peaks comparable with the normal endogenous nocturnal peaks. Conclusions: The authors conclude that melatonin concentrations during the day, comparable with plasma concentrations that occur spontaneously during sleep, do not suppress aqueous humor formation. The authors find no support for the idea that plasma melatonin, per se, can suppress aqueous formation or that the circadian rhythm of plasma melatonin is primarily responsible for the circadian rhythm of aqueous humor flow.
AB - Background: Aqueous humor flow through the anterior chamber of the eye undergoes a circadian cycle. The rate of flow during the day is twice as high as the rate of flow at night. The pineal hormone, melatonin, also undergoes a circadian cycle. Melatonin levels are high at night, whereas aqueous humor flow is low. The authors studied the effect of oral melatonin on aqueous humor flow in humans. Methods: The effect of melatonin on aqueous humor flow was evaluated in 19 healthy human volunteers in a randomized, masked crossover study with a placebo control. The hormone or placebo was administered orally during the day when endogenous levels of melatonin are low. Aqueous flow was measured by fluorophotometry for 8 hours. Results: The mean rate of flow during melatonin treatment was 2.71 ± 0.64 μl/minute (± standard deviation). The rate of flow during placebo treatment was 2.80 ± 0.66 μl/minute. There is no statistically significant difference between these two rates (P = 0.4). With a sample size of 19, the study has a power of 92% to detect at least a 15% difference in the rate of flow under the two conditions. Measurement of plasma concentration of melatonin in five subjects confirmed that concentrations after oral dosage reached peaks comparable with the normal endogenous nocturnal peaks. Conclusions: The authors conclude that melatonin concentrations during the day, comparable with plasma concentrations that occur spontaneously during sleep, do not suppress aqueous humor formation. The authors find no support for the idea that plasma melatonin, per se, can suppress aqueous formation or that the circadian rhythm of plasma melatonin is primarily responsible for the circadian rhythm of aqueous humor flow.
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U2 - 10.1016/S0161-6420(94)31332-7
DO - 10.1016/S0161-6420(94)31332-7
M3 - Article
C2 - 8115153
AN - SCOPUS:0028297481
SN - 0161-6420
VL - 101
SP - 326
EP - 331
JO - Ophthalmology
JF - Ophthalmology
IS - 2
ER -