The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum: Role of vagal innervation

Scott G. Houghton, Abdalla E. Zarroug, Judith A. Duenes, Martin E Fernandez-Zapico, Michael G. Sarr

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: Protein and messenger RNA (mRNA) levels of the hexose transporters sodium-dependent glucose transporter-1, glucose transporter 2, and glucose transporter 5 follow a (daily) diurnal rhythm in rat jejunum. Because vagal innervation mediates the diurnal activity of other proteins in the rat small bowel, we hypothesized that the diurnal variation of mRNA and protein levels of these hexose transport proteins are mediated by vagal innervation. Methods: Forty-eight rats kept in a strictly maintained, alternating 12-hour light-dark room underwent either sham laparotomy (n = 24) or bilateral total abdominal vagotomy (n = 24). Four weeks postoperatively, jejunal mucosa was harvested from 6 rats in each group at 3 am, 9 am, 3 pm and 9 pm; mRNA levels were determined by reverse transcription real-time polymerase chain reaction and protein levels by semiquantitative Western blot analysis. Transporter mRNA and protein levels were expressed as a ratio to the corresponding mRNA and protein levels of the stably expressed housekeeping gene glyceraldehyde-6-phosphate dehydrogenase. Results: mRNA and protein levels for all 3 hexose transporters showed diurnal variation in sham controls (P ≤ .01 for all). After vagotomy, although mRNA levels of all 3 transporters showed diurnal variation (each P < .01), diurnal variation in all 3 hexose transporter protein levels was abolished (P > .10 for all). Conclusions: Vagal innervation appears to differentially mediate the diurnal changes in hexose transporter mRNA and protein expression in the rat jejunum by posttranscriptional, and/or posttranslational processes.

Original languageEnglish (US)
Pages (from-to)542-549
Number of pages8
JournalSurgery
Volume139
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

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Monosaccharide Transport Proteins
Periodicity
Jejunum
Messenger RNA
Proteins
Facilitative Glucose Transport Proteins
Vagotomy
Sodium-Glucose Transporter 1
Glyceraldehyde
Essential Genes
Circadian Rhythm
Laparotomy
Reverse Transcription
Real-Time Polymerase Chain Reaction
Oxidoreductases
Mucous Membrane
Western Blotting
Phosphates
Light

ASJC Scopus subject areas

  • Surgery

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The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum : Role of vagal innervation. / Houghton, Scott G.; Zarroug, Abdalla E.; Duenes, Judith A.; Fernandez-Zapico, Martin E; Sarr, Michael G.

In: Surgery, Vol. 139, No. 4, 04.2006, p. 542-549.

Research output: Contribution to journalArticle

Houghton, Scott G. ; Zarroug, Abdalla E. ; Duenes, Judith A. ; Fernandez-Zapico, Martin E ; Sarr, Michael G. / The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum : Role of vagal innervation. In: Surgery. 2006 ; Vol. 139, No. 4. pp. 542-549.
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N2 - Background: Protein and messenger RNA (mRNA) levels of the hexose transporters sodium-dependent glucose transporter-1, glucose transporter 2, and glucose transporter 5 follow a (daily) diurnal rhythm in rat jejunum. Because vagal innervation mediates the diurnal activity of other proteins in the rat small bowel, we hypothesized that the diurnal variation of mRNA and protein levels of these hexose transport proteins are mediated by vagal innervation. Methods: Forty-eight rats kept in a strictly maintained, alternating 12-hour light-dark room underwent either sham laparotomy (n = 24) or bilateral total abdominal vagotomy (n = 24). Four weeks postoperatively, jejunal mucosa was harvested from 6 rats in each group at 3 am, 9 am, 3 pm and 9 pm; mRNA levels were determined by reverse transcription real-time polymerase chain reaction and protein levels by semiquantitative Western blot analysis. Transporter mRNA and protein levels were expressed as a ratio to the corresponding mRNA and protein levels of the stably expressed housekeeping gene glyceraldehyde-6-phosphate dehydrogenase. Results: mRNA and protein levels for all 3 hexose transporters showed diurnal variation in sham controls (P ≤ .01 for all). After vagotomy, although mRNA levels of all 3 transporters showed diurnal variation (each P < .01), diurnal variation in all 3 hexose transporter protein levels was abolished (P > .10 for all). Conclusions: Vagal innervation appears to differentially mediate the diurnal changes in hexose transporter mRNA and protein expression in the rat jejunum by posttranscriptional, and/or posttranslational processes.

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