The biochemical and histological effects of artificial ligament wear particles: In vitro and in vivo studies

E. J. Olson, J. D. Kang, F. H. Fu, H. I. Georgescu, G. C. Mason, Christopher H Evans

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Growing evidence suggests that biochemical mechanisms play a role in the pathogenesis of arthritis. Cartilaginous wear particles have been shown to induce destructive enzymes and cytokines. To assess the biocompatibility of artificial ACL replacements, the effects of wear particles from the following ligaments were analyzed biochemically and histologically: GORETEX, Stryker Dacron Ligament Prosthesis, Versigraft carbon, Kennedy LAD, Xenograft, Leeds-Keio, and human patellar tendon allograft. Ligaments were frozen and ground to produce wear particles similar to those seen clinically and were added to lapine synovial cell cultures. The resulting conditioned medium was analyzed for collagenase, gelatinase, and chondrocyte activating factor (CAF) production. All of the ligaments induced significantly elevated enzyme and CAF production by the synoviocytes, with Xenograft and carbon inducing significantly higher enzyme levels than those of the other five ligaments. Five milligrams of wear particles were injected into the knees of 4 kg to 5 kg rabbits that were analyzed histologically after 14 weeks. Wear particles accumulated in the periarticular synovial folds and induced modest to severe macrophage infiltration in the synovium. A hypothetical model explaining the role of artificial ligament wear particles in the pathogenesis of arthritis is presented.

Original languageEnglish (US)
Pages (from-to)558-570
Number of pages13
JournalAmerican Journal of Sports Medicine
Volume16
Issue number6
StatePublished - 1988
Externally publishedYes

Fingerprint

Ligaments
Chondrocytes
Heterografts
Arthritis
Enzymes
Carbon
Gelatinases
Patellar Ligament
Polyethylene Terephthalates
Synovial Membrane
Collagenases
Conditioned Culture Medium
Prostheses and Implants
Allografts
In Vitro Techniques
Knee
Cell Culture Techniques
Macrophages
Cytokines
Rabbits

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Public Health, Environmental and Occupational Health
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

The biochemical and histological effects of artificial ligament wear particles : In vitro and in vivo studies. / Olson, E. J.; Kang, J. D.; Fu, F. H.; Georgescu, H. I.; Mason, G. C.; Evans, Christopher H.

In: American Journal of Sports Medicine, Vol. 16, No. 6, 1988, p. 558-570.

Research output: Contribution to journalArticle

Olson, E. J. ; Kang, J. D. ; Fu, F. H. ; Georgescu, H. I. ; Mason, G. C. ; Evans, Christopher H. / The biochemical and histological effects of artificial ligament wear particles : In vitro and in vivo studies. In: American Journal of Sports Medicine. 1988 ; Vol. 16, No. 6. pp. 558-570.
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