The BBSome controls IFT assembly and turnaround in cilia

Qing Wei, Yuxia Zhang, Yujie Li, Qing Zhang, Kun Ling, Jinghua Hu

Research output: Contribution to journalArticle

127 Scopus citations

Abstract

The bidirectional movement of intraflagellar transport (IFT) particles, which are composed of motors, IFT-A and IFT-B subcomplexes, and cargoes, is required for the biogenesis and signalling of cilia. A successful IFT cycle depends on the proper assembly of the massive IFT particle at the ciliary base and its turnaround from anterograde to retrograde transport at the ciliary tip. However, how IFT assembly and turnaround are regulated in vivo remains elusive. From a whole-genome mutagenesis screen in Caenorhabditis elegans, we identified two hypomorphic mutations in dyf-2 and bbs-1 as the only mutants showing normal anterograde IFT transport but defective IFT turnaround at the ciliary tip. Further analyses revealed that the BBSome (refs,), a group of conserved proteins affected in human Bardet-Biedl syndrome (BBS), assembles IFT complexes at the ciliary base, then binds to the anterograde IFT particle in a DYF-2-(an orthologue of human WDR19) and BBS-1-dependent manner, and lastly reaches the ciliary tip to regulate proper IFT recycling. Our results identify the BBSome as the key player regulating IFT assembly and turnaround in cilia.

Original languageEnglish (US)
Pages (from-to)950-957
Number of pages8
JournalNature Cell Biology
Volume14
Issue number9
DOIs
StatePublished - Sep 2012

ASJC Scopus subject areas

  • Cell Biology

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