The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin Vκ-Jκ joining

Ryuji Hiramatsu, Kiwamu Akagi, Masao Matsuoka, Kunihiko Sakumi, Hiro Nakamura, Linda Kingsbury, Chella David, Richard R. Hardy, Ken ichi Yamamura, Hitoshi Sakano

Research output: Contribution to journalArticle

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Abstract

Using transgenic substrates, we found that the immunoglobulin κ gene 3′ enhancer (E3′) acts as a negative regulator in Vκ-Jκ joining. Although the E3′ was originally identified as a transcriptional enhancer, it acts in a Buppressive manner for recombinational regulation. Base substitution analysis has shown that the PU. 1-binding site within the E3′ regulates the B/T specificity of Vκ-Jκ joining. In a substrate with a mutated PU. 1-binding site (GAGGAA to TCTTCG), Vκ-Jκ joining occurred not only in B cells, but also in T cells. The E3′ region is also responsible for determining the pro-B/pre-B specificity of Vκ-Jκ joining. When the E3′ region was deleted, κ gene rearrangement actively occurred at the early pro-B stage of B cell development: non-germline (N) nucleotides were common at recombination junctions.

Original languageEnglish (US)
Pages (from-to)1113-1123
Number of pages11
JournalCell
Volume83
Issue number7
DOIs
StatePublished - Dec 29 1995

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Immunoglobulin Variable Region
Joining
B-Lymphocytes
Binding Sites
Immunoglobulin Genes
Gene Rearrangement
Genetic Recombination
Nucleotides
Genes
Cells
T-Lymphocytes
T-cells
Substrates
Immunoglobulins
Substitution reactions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Molecular Biology

Cite this

Hiramatsu, R., Akagi, K., Matsuoka, M., Sakumi, K., Nakamura, H., Kingsbury, L., ... Sakano, H. (1995). The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin Vκ-Jκ joining. Cell, 83(7), 1113-1123. https://doi.org/10.1016/0092-8674(95)90138-8

The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin Vκ-Jκ joining. / Hiramatsu, Ryuji; Akagi, Kiwamu; Matsuoka, Masao; Sakumi, Kunihiko; Nakamura, Hiro; Kingsbury, Linda; David, Chella; Hardy, Richard R.; Yamamura, Ken ichi; Sakano, Hitoshi.

In: Cell, Vol. 83, No. 7, 29.12.1995, p. 1113-1123.

Research output: Contribution to journalArticle

Hiramatsu, R, Akagi, K, Matsuoka, M, Sakumi, K, Nakamura, H, Kingsbury, L, David, C, Hardy, RR, Yamamura, KI & Sakano, H 1995, 'The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin Vκ-Jκ joining', Cell, vol. 83, no. 7, pp. 1113-1123. https://doi.org/10.1016/0092-8674(95)90138-8
Hiramatsu, Ryuji ; Akagi, Kiwamu ; Matsuoka, Masao ; Sakumi, Kunihiko ; Nakamura, Hiro ; Kingsbury, Linda ; David, Chella ; Hardy, Richard R. ; Yamamura, Ken ichi ; Sakano, Hitoshi. / The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin Vκ-Jκ joining. In: Cell. 1995 ; Vol. 83, No. 7. pp. 1113-1123.
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