TH‐C‐BRC‐01

Precision Localized Radiation to Ablate Progenitor Cells in the Mouse Brain

E. Ford, P. Achanta, M. Armour, J. Reyes, L. Kleinberg, Alfredo Quinones-Hinojosa

Research output: Contribution to journalArticle

Abstract

Purpose: Neural stem cells in the adult brain participate in both the repair of normal tissue and inhibition of tumor growth. Here we investigate the effects of localized irradiation on neural stem cells in the rodent subventricular zone (SVZ) emplying a novel device to precisely localize this stem cell niche. Method and Materials: We selectively targeted the SVZ of the mouse using a precision CT‐guided radiation device developed in our laboratory. We delivered 10 Gy to the right SVZ through a 3 mm field (n=4). The left SVZ serves as its own control, but sham mice were also employed (n=4). Mice were sacrificed 7 days after irradiation and brain tissue was harvested, fixed, frozen and sectioned. Immunohistochemical staining was performed for H2Ax, a marker of DNA double strand breaks, and Ki67, a marker of cell proliferation in this neurogenic region. Results: H2Ax stains indicated that the right lateral ventricle was specifically irradiated. A strong asymmetry was observed in the right (irradiated) vs. left (unirradiated) SVZ with 14,106±626 vs. 2,323±1094 cells (p<0.001). The data also showed that the unirradiated (left) SVZ has the same number of Ki67+ cells as a sham mouse (p=0.27) indicating that local irradiation produces no systemic effect under these conditions. Conclusion: Localized irradiation is capable of selectively ablating proliferating cells in neurogenic regions of the mouse brain. This local dose response as well as the irradiating device can be used to study locoregional effects and migration of neural stem cells.

Original languageEnglish (US)
Pages (from-to)2797-2798
Number of pages2
JournalMedical Physics
Volume36
Issue number6
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Lateral Ventricles
Stem Cells
Radiation
Brain
Neural Stem Cells
Equipment and Supplies
Stem Cell Niche
Double-Stranded DNA Breaks
Heart Ventricles
Rodentia
Coloring Agents
Cell Count
Cell Proliferation
Staining and Labeling
Growth
Neoplasms

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging

Cite this

TH‐C‐BRC‐01 : Precision Localized Radiation to Ablate Progenitor Cells in the Mouse Brain. / Ford, E.; Achanta, P.; Armour, M.; Reyes, J.; Kleinberg, L.; Quinones-Hinojosa, Alfredo.

In: Medical Physics, Vol. 36, No. 6, 2009, p. 2797-2798.

Research output: Contribution to journalArticle

Ford, E. ; Achanta, P. ; Armour, M. ; Reyes, J. ; Kleinberg, L. ; Quinones-Hinojosa, Alfredo. / TH‐C‐BRC‐01 : Precision Localized Radiation to Ablate Progenitor Cells in the Mouse Brain. In: Medical Physics. 2009 ; Vol. 36, No. 6. pp. 2797-2798.
@article{5132a091210a43278d8cd61007dda326,
title = "TH‐C‐BRC‐01: Precision Localized Radiation to Ablate Progenitor Cells in the Mouse Brain",
abstract = "Purpose: Neural stem cells in the adult brain participate in both the repair of normal tissue and inhibition of tumor growth. Here we investigate the effects of localized irradiation on neural stem cells in the rodent subventricular zone (SVZ) emplying a novel device to precisely localize this stem cell niche. Method and Materials: We selectively targeted the SVZ of the mouse using a precision CT‐guided radiation device developed in our laboratory. We delivered 10 Gy to the right SVZ through a 3 mm field (n=4). The left SVZ serves as its own control, but sham mice were also employed (n=4). Mice were sacrificed 7 days after irradiation and brain tissue was harvested, fixed, frozen and sectioned. Immunohistochemical staining was performed for H2Ax, a marker of DNA double strand breaks, and Ki67, a marker of cell proliferation in this neurogenic region. Results: H2Ax stains indicated that the right lateral ventricle was specifically irradiated. A strong asymmetry was observed in the right (irradiated) vs. left (unirradiated) SVZ with 14,106±626 vs. 2,323±1094 cells (p<0.001). The data also showed that the unirradiated (left) SVZ has the same number of Ki67+ cells as a sham mouse (p=0.27) indicating that local irradiation produces no systemic effect under these conditions. Conclusion: Localized irradiation is capable of selectively ablating proliferating cells in neurogenic regions of the mouse brain. This local dose response as well as the irradiating device can be used to study locoregional effects and migration of neural stem cells.",
author = "E. Ford and P. Achanta and M. Armour and J. Reyes and L. Kleinberg and Alfredo Quinones-Hinojosa",
year = "2009",
doi = "10.1118/1.3182615",
language = "English (US)",
volume = "36",
pages = "2797--2798",
journal = "Medical Physics",
issn = "0094-2405",
publisher = "AAPM - American Association of Physicists in Medicine",
number = "6",

}

TY - JOUR

T1 - TH‐C‐BRC‐01

T2 - Precision Localized Radiation to Ablate Progenitor Cells in the Mouse Brain

AU - Ford, E.

AU - Achanta, P.

AU - Armour, M.

AU - Reyes, J.

AU - Kleinberg, L.

AU - Quinones-Hinojosa, Alfredo

PY - 2009

Y1 - 2009

N2 - Purpose: Neural stem cells in the adult brain participate in both the repair of normal tissue and inhibition of tumor growth. Here we investigate the effects of localized irradiation on neural stem cells in the rodent subventricular zone (SVZ) emplying a novel device to precisely localize this stem cell niche. Method and Materials: We selectively targeted the SVZ of the mouse using a precision CT‐guided radiation device developed in our laboratory. We delivered 10 Gy to the right SVZ through a 3 mm field (n=4). The left SVZ serves as its own control, but sham mice were also employed (n=4). Mice were sacrificed 7 days after irradiation and brain tissue was harvested, fixed, frozen and sectioned. Immunohistochemical staining was performed for H2Ax, a marker of DNA double strand breaks, and Ki67, a marker of cell proliferation in this neurogenic region. Results: H2Ax stains indicated that the right lateral ventricle was specifically irradiated. A strong asymmetry was observed in the right (irradiated) vs. left (unirradiated) SVZ with 14,106±626 vs. 2,323±1094 cells (p<0.001). The data also showed that the unirradiated (left) SVZ has the same number of Ki67+ cells as a sham mouse (p=0.27) indicating that local irradiation produces no systemic effect under these conditions. Conclusion: Localized irradiation is capable of selectively ablating proliferating cells in neurogenic regions of the mouse brain. This local dose response as well as the irradiating device can be used to study locoregional effects and migration of neural stem cells.

AB - Purpose: Neural stem cells in the adult brain participate in both the repair of normal tissue and inhibition of tumor growth. Here we investigate the effects of localized irradiation on neural stem cells in the rodent subventricular zone (SVZ) emplying a novel device to precisely localize this stem cell niche. Method and Materials: We selectively targeted the SVZ of the mouse using a precision CT‐guided radiation device developed in our laboratory. We delivered 10 Gy to the right SVZ through a 3 mm field (n=4). The left SVZ serves as its own control, but sham mice were also employed (n=4). Mice were sacrificed 7 days after irradiation and brain tissue was harvested, fixed, frozen and sectioned. Immunohistochemical staining was performed for H2Ax, a marker of DNA double strand breaks, and Ki67, a marker of cell proliferation in this neurogenic region. Results: H2Ax stains indicated that the right lateral ventricle was specifically irradiated. A strong asymmetry was observed in the right (irradiated) vs. left (unirradiated) SVZ with 14,106±626 vs. 2,323±1094 cells (p<0.001). The data also showed that the unirradiated (left) SVZ has the same number of Ki67+ cells as a sham mouse (p=0.27) indicating that local irradiation produces no systemic effect under these conditions. Conclusion: Localized irradiation is capable of selectively ablating proliferating cells in neurogenic regions of the mouse brain. This local dose response as well as the irradiating device can be used to study locoregional effects and migration of neural stem cells.

UR - http://www.scopus.com/inward/record.url?scp=85024812218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85024812218&partnerID=8YFLogxK

U2 - 10.1118/1.3182615

DO - 10.1118/1.3182615

M3 - Article

VL - 36

SP - 2797

EP - 2798

JO - Medical Physics

JF - Medical Physics

SN - 0094-2405

IS - 6

ER -